Amino Acid Profile Determination in the Urine of Bladder Cancer Patients by CE-MS with On-Line pH-Mediated Stacking and Pattern Recognition

被引：19

作者：

Wang, Shufang ^{[2
]}

Yang, Ping ^{[2
]}

Zhao, Xiaoping ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Coll Preclin Med, Hangzhou 310053, Zhejiang, Peoples R China

[2] Zhejiang Univ, Pharmaceut Informat Inst, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CHROMATOGRAPHIA | 2009年 / 70卷 / 9-10期

关键词：

Capillary electrophoresis-mass spectrometry; pH-Mediated stacking; Pattern recognition; Bladder cancer; Amino acid in urine; CAPILLARY-ZONE-ELECTROPHORESIS; IONIZATION-MASS-SPECTROMETRY; METABOLITES; MARKERS; PLASMA;

D O I：

10.1365/s10337-009-1319-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive capillary electrophoresis-mass spectrometry with on-line pH-mediated stacking method for the simultaneous quantification of 19 amino acids in urine was established and applied for the analysis of the urinary amino acids of 20 bladder cancer patients and 20 healthy subjects. The two groups could be well classified by pattern recognition, which indicated that the two groups had different urinary amino acids profiles. Compared to those of healthy subjects, the relative concentrations of three glycogenic amino acids (methionine, cysteine and valine) decreased significantly in the urine of bladder cancer patients.

引用

页码：1479 / 1484

页数：6

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