Evaluation of potential probiotics properties of the screened Lactobacilli isolated from home-made koumiss in Mongolia

被引:19
作者
Bilige, Menghe [1 ]
Liu, Wenjun [1 ]
Rina, Wu [1 ]
Wang, Liping [1 ]
Sun, Tiansong [1 ]
Wang, Junguo [1 ]
Li, Haiping [2 ]
Zhang, Heping [1 ]
机构
[1] Inner Mongolia Agr Univ, Educ Minist China, Key Lab Dairy Biotechnol & Bioengn, Hohhot, Peoples R China
[2] Inner Mongolia Agr Univ, Dept Food Sci & Engn, Hohhot, Peoples R China
关键词
koumiss; Lactobacillus; cholesterol removal; adhesiveness; probiotics; BILE-SALT DECONJUGATION; CHOLESTEROL REMOVAL; ASSIMILATION; TOLERANCE; STRAINS; ACIDOPHILUS; BACTERIA; MEDIA;
D O I
10.1007/BF03175136
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Among thirty Lactobacillus strains, which were isolated and identified from the home-made koumiss in Mongolia, Lactobacillus helveticus MG2-1 was screened out for the best acid tolerance in artificial gastric juice at pH 3.0 after incubating for 3 h with the survival rate 96.15 +/- 4.22%. Further in vitro assessment revealed MG2-1 showed high tolerance to the artificial gastrointestinal juice and bile salts. Moreover, other desired probiotic proper-ties, including the ability of sodium taurocholate deconjugation (with the amount of cholic acid liberated 2.95 +/- 0.11 mu M), cholesterol removal (cholesterol removal rate 51.74 +/- 0.04%) and adhesion to Caco-2 cells (the adhesive rate 1.51 +/- 0.11%), were also observed. The results highlighted the probiotic potential of MG2-1, which deserved further investigation in in vivo studies to elucidate its health benefits.
引用
收藏
页码:493 / 498
页数:6
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