Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

被引：26

作者：

Wang, Li-Juan ^{[1
]}

Wang, Zi-Yue ^{[1
]}

Zhang, Qianyi ^{[2
]}

Tang, Bo ^{[1
]}

Zhang, Chun-Yang ^{[1
]}

机构：

[1] Univ Shandong, Coll Chem Chem Engn & Mat Sci,Minist Educ, Collaborat Innovat Ctr Functionalized Probes Chem, Key Lab Mol & Nano Probes,Shandong Prov Key Lab, Jinan 250014, Peoples R China

[2] Nantou High Sch Shenzhen, Shenzhen 518052, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 27期

基金：

中国国家自然科学基金;

关键词：

BASE-EXCISION-REPAIR; ULTRASENSITIVE DETECTION; INDUCED CHEMILUMINESCENCE; SENSITIVE DETECTION; STRUCTURAL BASIS; MAMMALIAN DNA; 5-CARBOXYLCYTOSINE; DEMETHYLATION; RECEPTORS; ASSAY;

D O I：

10.1039/c7cc00946a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase (TDG) activity through cyclic enzymatic repairing-mediated dual-signal amplification. This method exhibits excellent sensitivity with a detection limit of 5.6 x 10(-7) U mu L-1, and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells.

引用

页码：3878 / 3881

页数：4

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