Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

被引：26
作者：
Wang, Li-Juan [1 ]
Wang, Zi-Yue [1 ]
Zhang, Qianyi [2 ]
Tang, Bo [1 ]
Zhang, Chun-Yang [1 ]
机构：
[1] Univ Shandong, Coll Chem Chem Engn & Mat Sci,Minist Educ, Collaborat Innovat Ctr Functionalized Probes Chem, Key Lab Mol & Nano Probes,Shandong Prov Key Lab, Jinan 250014, Peoples R China
[2] Nantou High Sch Shenzhen, Shenzhen 518052, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 27期
基金：
中国国家自然科学基金;
关键词：
BASE-EXCISION-REPAIR; ULTRASENSITIVE DETECTION; INDUCED CHEMILUMINESCENCE; SENSITIVE DETECTION; STRUCTURAL BASIS; MAMMALIAN DNA; 5-CARBOXYLCYTOSINE; DEMETHYLATION; RECEPTORS; ASSAY;
D O I：
10.1039/c7cc00946a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase (TDG) activity through cyclic enzymatic repairing-mediated dual-signal amplification. This method exhibits excellent sensitivity with a detection limit of 5.6 x 10(-7) U mu L-1, and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells.
引用
收藏
页码：3878 / 3881
页数：4
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