Spectroscopic Investigation Of Hemoglobin Adsorbed Onto Gold Nanoparticles

被引：0

作者：

Garabagiu, Sorina ^{[1
]}

Mihailescu, Gheorghe ^{[1
]}

机构：

[1] Natl Inst Res & Dev Isotop & Mol Technol, Cluj Napoca 400293, Romania

来源：

PROCESSES IN ISOTOPES AND MOLECULES (PIM 2011) | 2012年 / 1425卷

关键词：

Gold nanoparticles; Hemoglobin; Fluorescence spectroscopy; FLUORESCENCE;

D O I：

10.1063/1.3681982

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The adsorption of horse hemoglobin onto the surface of gold nanoparticles was studied using fluorescence spectroscopy. Gold nanoparticles quench the fluorescence emission of tryptophan residues in the structure of hemoglobin. By representing the dependence of log[(F-0-F)/(F-F-sat)] on the logarithm of the concentration of gold nanoparticles in the solution, we obtained the binding parameters of hemoglobin onto the surface of gold nanoparticles. The number of binding sites was calculated to be 1.181 and the binding constant 7.935*10(9) M-1

引用

页码：124 / 126

页数：3

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