Indirect somatic embryogenesis in Coffea with different ploidy levels: a revisiting and updating study

被引:15
作者
Sanglard, Natalia Arruda [1 ]
Amaral-Silva, Paulo Marcos [1 ]
Sattler, Mariana Cansian [2 ]
de Oliveira, Stefanie Cristina [1 ]
Cesario, Leticia Miranda [1 ]
Ferreira, Adesio [3 ]
Carvalho, Carlos Roberto [2 ]
Clarindo, Wellington Ronildo [2 ]
机构
[1] Univ Fed Espirito Santo, Dept Biol, Lab Citogenet & Cultura Tecidos Vegetais, Campus Alegre, BR-29500000 Alegre, ES, Brazil
[2] Univ Fed Vicosa, Ctr Ciencias Biol & Saude, Dept Biol Geral, Lab Citogenet & Citometria, BR-36570000 Vicosa, MG, Brazil
[3] Univ Fed Espirito Santo, Dept Agron, Lab Biometria, Campus Alegre, BR-29500000 Alegre, ES, Brazil
关键词
Coffee; Plant tissue culture; In vitro somatic embryogenesis; Euploidy; Chromosome number; Nuclear 2C value; PLANT-GROWTH REGULATORS; CULTURE; TISSUE; CELLS; HIBRIDO; ORIGIN;
D O I
10.1007/s11240-018-1511-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Indirect somatic embryogenesis (ISE) is required for plant propagation and a prerequisite for applications that may provide new germplasms. Genetic, epigenetic and physiological features of the explant donor are barriers for ISE establishment, hindering its wide use. Despite the identification and/or expression analysis of genes during ISE, no approach to establish the karyotype aspects has been performed so far. So, this study aims to establish the ISE and compare the in vitro responses between diploid (Coffea canephora and Coffea eugenioides), allotriploid (Hibrido de TimorHT) and true allotetraploid (Coffea arabica) Coffea in a taxonomic and evolutive scenario. Under the same in vitro conditions, the four Coffea differed from each other during ISE. Leaf explants of the true allopolyploids yielded the highest mean number of friable calli (FC) in relative short time and visually exhibiting more pronounced length. FC of the allotetraploid C. arabica presented the highest mean number of mature cotyledonary somatic embryos (MCSE), which were also recovered faster in this species. However, MCSE mean number in HT was the same or lower than diploid Coffea. Besides, intraspecific variation related to the ISE responses was observed in each Coffea, mainly the mean number of FC obtained from ex vitro and in vitro C. arabica and C. eugenioides explants. So, epigenetic and physiologic features may also have influenced the ISE responses. The findings provide the basis for performing other approaches considering the ploidy level, epigenetic and physiological backgrounds. Besides, the data also contributed for understanding about the consequences of polyploidy.
引用
收藏
页码:255 / 267
页数:13
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