Determination of the degrees of methylation and acetylation of pectins using a C18 column and internal standards

被引：154

作者：

Levigne, S ^{[1
]}

Thomas, M ^{[1
]}

Ralet, MC ^{[1
]}

Quemener, B ^{[1
]}

Thibault, JF ^{[1
]}

机构：

[1] INRA, Unite Rech Polysaccharides Org & Interact, F-44316 Nantes 03, France

来源：

FOOD HYDROCOLLOIDS | 2002年 / 16卷 / 06期

关键词：

pectin; degree of esterification; methanol; acetic acid;

D O I：

10.1016/S0268-005X(02)00015-2

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An improved hplc method for the simultaneous determination of the degrees of methylation and acetylation of pectins has been developed. The proposed method involves saponification in heterogeneous medium followed by the separation of methanol, acetic acid and internal standard on a C18 column and quantification by refractometry. The method is reproducible, accurate, and presents several advantages that are discussed in the present paper. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：547 / 550

页数：4

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