Analysis and Quantitation of Glycated Hemoglobin by Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry

被引:21
作者
Hattan, Stephen J. [1 ]
Parker, Kenneth C. [1 ]
Vestal, Marvin L. [1 ]
Yang, Jane Y. [2 ]
Herold, David A. [2 ,3 ]
Duncan, Mark W. [4 ]
机构
[1] SimulTOF Syst, Sudbury, MA 01776 USA
[2] Univ Calif San Diego, Dept Pathol, La Jolla, CA 92093 USA
[3] VA San Diego Healthcare Syst, PALMS, MS 113, San Diego, CA 92161 USA
[4] Univ Colorado, Sch Med, Div Endocrinol Metab & Diabet, MS 8106, Aurora, CO 80045 USA
关键词
Hemoglobin; Matrix assisted laser desorption ionization; MALDI-TOF; Diabetes; Glycation; Quantitation; DIABETES-MELLITUS; CAPILLARY-ELECTROPHORESIS; LIFE-STYLE; DISEASE; A(1C); CHROMATOGRAPHY; MORTALITY; COLUMN; ASSAY;
D O I
10.1007/s13361-015-1316-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Measurement of glycated hemoglobin is widely used for the diagnosis and monitoring of diabetes mellitus. Matrix assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometry (MS) analysis of patient samples is used to demonstrate a method for quantitation of total glycation on the beta-subunit of hemoglobin. The approach is accurate and calibrated with commercially available reference materials. Measurements were linear (R-2 > 0.99) across the clinically relevant range of 4% to 20% glycation with coefficients of variation of a parts per thousand currency sign 2.5%. Additional and independent measurements of glycation of the alpha-subunit of hemoglobin are used to validate beta-subunit glycation measurements and distinguish hemoglobin variants. Results obtained by MALDI-TOF MS were compared with those obtained in a clinical laboratory using validated HPLC methodology. MALDI-TOF MS sample preparation was minimal and analysis times were rapid making the method an attractive alternative to methodologies currently in practice.
引用
收藏
页码:532 / 541
页数:10
相关论文
共 43 条
[1]  
American Diabetes Association, 2011, DIABETES CARE, V35, P11
[2]  
[Anonymous], 2014, Global health estimates: deaths by cause, age, sex and country, 2000-2012
[3]  
[Anonymous], 2014, GLOBAL STATUS REPORT
[4]  
[Anonymous], DIAB TESTS DIAGN
[5]  
Ashley-Koch A, 2000, AM J EPIDEMIOL, V151, P839
[6]   A quantitative method for the analysis of glycated and glutathionylated hemoglobin by matrix-assisted laser desorption ionization-time of flight mass spectrometry [J].
Biroccio, A ;
Urbani, A ;
Massoud, R ;
di Ilio, C ;
Sacchetta, P ;
Bernardini, S ;
Cortese, C ;
Federici, G .
ANALYTICAL BIOCHEMISTRY, 2005, 336 (02) :279-288
[7]   STRUCTURE OF HEMOGLOBIN AIC - NATURE OF N-TERMINAL BETA CHAIN BLOCKING GROUP [J].
BOOKCHIN, RM ;
GALLOP, PM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 32 (01) :86-&
[8]   International Hemoglobin Information Center variant list - Introduction [J].
Carver, MFH ;
Huisman, THJ .
HEMOGLOBIN, 1996, 20 (03) :213-213
[9]   DETERMINATION OF THE NONENZYMATIC GLYCATION OF HEMOGLOBIN BY ISOELECTROFOCUSING OF ITS GLOBIN CHAINS [J].
CASTAGNOLA, M ;
CARADONNA, P ;
BERTOLLINI, A ;
CASSIANO, L ;
ROSSETTI, DV ;
SALVI, ML .
CLINICAL BIOCHEMISTRY, 1985, 18 (06) :327-331
[10]   IMPROVED METHOD FOR ANALYSIS OF GLYCATED HEMOGLOBIN BY ION-EXCHANGE CHROMATOGRAPHY [J].
ECKERBOM, S ;
BERGQVIST, Y ;
JEPPSSON, JO .
ANNALS OF CLINICAL BIOCHEMISTRY, 1994, 31 :355-360