Enantiomer separation of denopamine by capillary electrophoresis with charged and uncharged cyclodextrins

被引：18

作者：

Ishibuchi, K ^{[1
]}

Izumoto, S ^{[1
]}

Nishi, H ^{[1
]}

Sato, T ^{[1
]}

机构：

[1] TANABE SEIYAKU CO LTD,ANALYT RES LAB,YODOGAWA KU,OSAKA 532,JAPAN

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 06期

关键词：

enantiomer separation; denopamine; capillary electrophoresis; charged cyclodextrins;

D O I：

10.1002/elps.1150180624

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct separation of enantiomers of denopamine was investigated by capillary electrophoresis employing charged and uncharged cyclodextrin (CD) derivatives. Uncharged beta-type CDs, having hydrophobic groups, were essential for the enantioseparation of denopamine; of these, especially dimethyl-beta-CD was effective. Among charged CDs, gamma-type as well as beta-type CDs were found effective for the enantioseparation of denopamine. Reversal of migration order of R-form (active) and S-form enantiomers was investigated by using two types of coated capillaries: (i) an amine capillary with an inner wall coated with dimethylamino groups, and (ii) a polyacrylamide-coated capillary. Manipulation of migration order could be easily performed by selecting suitable capillaries, buffer pH, and CDs.

引用

页码：1007 / 1012

页数：6

共 23 条

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