Current and future editing reagent delivery systems for plant genome editing

被引:83
作者
Ran, Yidong [1 ]
Liang, Zhen [2 ,3 ]
Gao, Caixia [2 ]
机构
[1] Genovo Biotechnol Co Ltd, Tianjin 301700, Peoples R China
[2] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
关键词
genome editing; DNA; RNA; RNP and virus delivery; Agrobacterium-mediated transformation; biolistic method; protoplast transfection; ttransgene-free genome edited plant; EFFICIENT TARGETED MUTAGENESIS; ZINC-FINGER NUCLEASES; MESOPOROUS SILICA NANOPARTICLES; SITE-SPECIFIC MUTAGENESIS; HOMOLOGOUS RECOMBINATION; NICOTIANA-BENTHAMIANA; CRISPR/CAS9; SYSTEM; CROP PLANTS; DIRECTED MUTAGENESIS; HERITABLE MUTATIONS;
D O I
10.1007/s11427-017-9022-1
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have opened up a new era for crop improvement due to their precise editing of user-specified sequences related to agronomic traits. In this review, we will focus on an update of recent developments in the methodologies of editing reagent delivery, and consider the pros and cons of current delivery systems. Finally, we will reflect on possible future directions.
引用
收藏
页码:490 / 505
页数:16
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