Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study

被引：48

作者：

Reed, Michael L. ^{[1
]}

Hamic, Amanda ^{[1
]}

Thompson, Douglas J. ^{[1
]}

Caperton, Charles L. ^{[1
]}

机构：

[1] Ctr Reprod Med New Mexico, Albuquerque, NM 87106 USA

来源：

FERTILITY AND STERILITY | 2009年 / 92卷 / 05期

关键词：

IN-VITRO FERTILIZATION; PREIMPLANTATION EMBRYOS; PREGNANCY; IMPLANTATION; VIABILITY; AMMONIUM; CLEAVAGE;

D O I：

10.1016/j.fertnstert.2009.05.008

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Eight hundred ninety-three sibling embryos from 80 IVF cycles were cultured side by side in either: 1) a single medium continuously, without medium renewal on day 3; or 2) sequential media. There were no significant differences between the two culture media systems regarding embryo quality or the proportion of embryos selected for transfer on day 3 from either media; however, for day 5 embryo transfer, a greater number of blastocysts were available, and were selected for transfer, from the continuous single medium culture compared with sequential media culture. (Fertil Steril (R) 2009;92:1783-6. (C) 2009 by American Society for Reproductive Medicine.)

引用

页码：1783 / 1786

页数：4

共 22 条

[1]

ANGLE MJ, 2006, CLIN EMBRYOL, V9, P5

[2] Culture of preimplantation embryos: Facts and artifacts [J].

Bavister, BD .

HUMAN REPRODUCTION UPDATE, 1995, 1 (02) :91-148

[3] Interactions between embryos and the culture milieu [J].

Bavister, BD .

THERIOGENOLOGY, 2000, 53 (02) :619-626

[4] Choosing a culture medium: making informed choices [J].

Biggers, John D. ;

Summers, Michael C. .

FERTILITY AND STERILITY, 2008, 90 (03) :473-483

[5]

Biggers John D, 2002, Reprod Biomed Online, V5, P133

[6] Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer [J].

Gardner, DK ;

Lane, M ;

Stevens, J ;

Schlenker, T ;

Schoolcraft, WB .

FERTILITY AND STERILITY, 2000, 73 (06) :1155-1158

[7] Medium without Ammonium Accumulation Supports the Developmental Competence of Human Embryos [J].

Hashimoto, Shu ;

Nishihara, Takuji ;

Murata, Yasutaka ;

Oku, Hirotsugu ;

Nakaoka, Yoshiharu ;

Fukuda, Aisaku ;

Morimoto, Yoshiharu .

JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2008, 54 (05) :370-374

[8] Implantation rates after in vitro fertilization and transfer of a maximum of two embryos that have undergone three to five days of culture [J].

Huisman, GJ ;

Fauser, BCJM ;

Eijkemans, MJC ;

Pieters, MHEC .

FERTILITY AND STERILITY, 2000, 73 (01) :117-122

[9] Understanding cellular disruptions during early embryo development that perturb viability and fetal development [J].

Lane, M ;

Gardner, DK .

REPRODUCTION FERTILITY AND DEVELOPMENT, 2005, 17 (03) :371-378

[10] Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse [J].

Lane, M ;

Gardner, DK .

BIOLOGY OF REPRODUCTION, 2003, 69 (04) :1109-1117

← 1 2 3 →