CRISPR base editing applications for identifying cancer-driving mutations

被引：7

作者：

Pal, Martin ^{[1
,2
]}

Herold, Marco J. ^{[1
,2
]}

机构：

[1] WEHI Walter & Eliza Hall Inst, Melbourne, Vic 3052, Australia

[2] Univ Melbourne, Dept Med Biol, Melbourne, Vic 3052, Australia

来源：

BIOCHEMICAL SOCIETY TRANSACTIONS | 2021年 / 49卷 / 01期

基金：

英国医学研究理事会;

关键词：

METABOLIC LIVER-DISEASE; GENOMIC DNA; COMPLEX; RICE;

D O I：

10.1042/BST20200550

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations and (ii) it avoids creating DNA double-strand breaks, which can lead to unintended chromosomal alterations or elicit an unwanted DNA damage response. Given many cancers originate from point mutations in cancer-driving genes, the application of base editing for either modelling tumour development, therapeutic editing, or functional screening is of great promise. In this review, we summarise current DNA base editing technologies and will discuss recent advancements and existing hurdles for its usage in cancer research.

引用

页码：269 / 280

页数：12

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