Axon- or dendrite-predominant outgrowth induced by constituents from Ashwagandha

被引:83
作者
Kuboyama, T
Tohda, C
Zhao, J
Nakamura, N
Hattori, M
Komatsu, K
机构
[1] Toyama Med & Pharmaceut Univ, Res Ctr Ethnomed, Toyama 9300194, Japan
[2] Toyama Med & Pharmaceut Univ, Dept Metab Engn, Inst Nat Med, Toyama 9300194, Japan
关键词
Ashwagandha; axon; dendrite; Withania somnifera; withanolide A; withanoside IV; withanoside VI;
D O I
10.1097/00001756-200210070-00005
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We previously reported that the methanol extract of Ashwagandha (roots of Withania somnifera Dunal) induced dendrite extension in a human neuroblastoma cell line. In this study, we found that six of the 18 compounds isolated from the methanol extract enhanced neurite outgrowth in human neuroblastoma SH-SY5Y cells. Double immunostaining was performed in rat cortical neurons using antibodies to phosphorylated NF-H as an axonal marker, and to MAP2 as a dendritic marker. In withanolide A-treated cells, the length of NF-H-positive processes was significantly increased compared with vehicle-treated cells, whereas, the length of MAP2-positive processes was increased by withanosides IV and VI. These results suggest that axons are predominantly extended by withanolide A, and dendrites by withanosides IV and VI. (C) 2002 Lippincott Williams Wilkins.
引用
收藏
页码:1715 / 1720
页数:6
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