Multi-omics analysis of aniline-degrading bacterium, Delftia sp. K82

被引：6

作者：

Lee, Sang-Yeop ^{[1
]}

Yun, Sung Ho ^{[2
]}

Lee, Hayoung ^{[1
,3
]}

Seo, Giwan ^{[1
]}

Kim, Seung Il ^{[1
,3
]}

机构：

[1] Korea Basic Sci Inst, Res Ctr Bioconvergence Anal, Ochang 28119, South Korea

[2] Korea Basic Sci Inst, Ctr Res Equipment, Ochang 28119, South Korea

[3] Univ Sci & Technol UST, Dept Bioanalyt Sci, Daejeon 34113, South Korea

来源：

JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY | 2021年 / 12卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Delftia sp; K82; Genomics; Transcriptomics; Proteomics; Biodegradation; Aniline;

D O I：

10.1186/s40543-021-00258-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Delftia sp. K82 is a soil bacterium capable of utilizing monocyclic aromatic hydrocarbons, including aniline, as its sole carbon and nitrogen source. In this study, the genome analysis of Delftia sp. K82 was completed and the genome data (6117 protein-coding genes and 62 RNA genes) were utilized for proteomic and transcriptomic analysis of Delftia sp. K82 cultured in aniline culture medium. Using these multi-omics approaches (genomics, transcriptomics, and proteomics), complete gene clusters for aniline biodegradation pathways were identified and transcriptomic or proteomic sets specifically induced in aniline culture conditions were elucidated. These data provide multi-layered information on the metabolic characteristics of Delftia sp. K82. The findings suggest that multi-omics approaches are useful analytical tools for the elucidation of the metabolic diversity of soil bacteria and for the identification of novel metabolic enzymes.

引用

页数：7