PROTEIN-CAPPED GOLD NANOPARTICLES OBTAINED BY A GREEN SYNTHESIS METHOD

被引:0
作者
Coman, Cristina [1 ]
Leopold, Loredana Florina [1 ]
Rugina, Olivia-Dumitrita [1 ]
Diaconeasa, Zorita [1 ]
Bolfa, Pompei Florin [2 ]
Leopold, Nicolae [3 ]
Tofana, Maria [1 ]
Socaciu, Carmen [1 ]
机构
[1] Univ Agr Sci & Vet Med, Fac Food Sci & Technol, Manastur 3-5, Cluj Napoca 400372, Romania
[2] Univ Agr Sci & Vet Med, Fac Vet Med, Cluj Napoca 400372, Romania
[3] Univ Babes Bolyai, Fac Phys, Cluj Napoca 400084, Romania
来源
STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA | 2015年 / 60卷 / 01期
关键词
gold nanoparticles; FTIR; TEM; HFL-1; cells; BIOLOGICAL SYNTHESIS; METAL NANOPARTICLES; LEAF EXTRACT; SILVER; BIOSYNTHESIS;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A green synthesis protocol for the synthesis of colloidal gold nanoparticles is proposed. The synthesis involves the use of garlic (Allium sativum) extract as reducing agent. The reduction is carried out by slow addition of the Allium sativum extract to the chloroauric acid at boiling and gives spherically shaped nanoparticles, with 10-20 nm size distribution and mean diameters of 15 nm. The gold nanoparticles are characterized by UV-VIS spectroscopy, Fourier Transform Infrared Spectroscopy, Transmission Electron Microscopy, and Surface-Enhanced Raman Scattering. In vitro tests are also performed on human fetal lung fibroblast HFL-1 cells and the nanoparticles are found to be non cytotoxic for the cells in the concentration range used in our study. Confocal microscopy shows that the particles are localised in the cell cytoplasm.
引用
收藏
页码:119 / 126
页数:8
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