Chondrogenesis of human periosteum-derived progenitor cells in atelocollagen

被引：32
作者：
Choi, Yong-Soo
Lim, Sang-Min
Shin, Hyun-Chong
Lee, Chang-Woo
Kim, Sang-Lin
Kim, Dong-Il [1 ]
机构：
[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea
[2] Good Shepherd Hosp, Seoul 135717, South Korea
[3] Boryung Pharmaceut Co Ltd, Ansan 425120, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 02期
关键词：
atelocollagen; cartilage; chondrogenesis; periosteum-derived progenitor cells;
D O I：
10.1007/s10529-006-9240-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Periosteum-derived progenitor cells (PDPCs) could be differentiated into cartilage using atelocollagen as a carrier and in the presence of transforming growth factor-b3 (TGF-b3). Chondrogenesis was verified by RT-PCR and Western blotting. Expression of the type II collagen mRNA was found from the differentiated PDPCs in atelocollagen 3 weeks after chondrogenic induction. The chondrogenic potential of the PDPCs was also verified by histochemical staining for type II collagen protein. Increased production of glycosaminoglycan shows that the PDPCs in atelocollagen could differentiate into chondrocytes under a chondrogenic environment. PDPCs can therefore be used as a cell source for cell-based therapies targeted toward the articular cartilage of the knee.
引用
收藏
页码：323 / 329
页数：7
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