Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells

被引：5

作者：

Kwak, Dong Hoon ^{[3
]}

Jin, Jung-Woo ^{[2
]}

Ryu, Jae-Sung ^{[2
]}

Ko, Kinram ^{[4
]}

Lee, So-Dam ^{[2
]}

Lee, Jeong-Woong ^{[5
]}

Kim, Ji-Su ^{[1
]}

Jung, Kyu Yong ^{[6
]}

Ko, Kisung ^{[2
]}

Ma, Jin Yeul ^{[3
]}

Hwang, Kyung-A ^{[7
]}

Chang, Kyu-Tae ^{[1
]}

Choo, Young-Kug ^{[2
]}

机构：

[1] Korea Inst Biosci & Biotechnol, Natl Primate Res Ctr, O Chang 363883, South Korea

[2] Wonkwang Univ, Dept Biol Sci, Coll Nat Sci, Iksan 570749, South Korea

[3] Korea Inst Oriental Med, Ctr Herbal Med Improvement Res, Taejon 305811, South Korea

[4] Konkuk Univ, Sch Med, Dept Neurosci, Seoul 143701, South Korea

[5] Korea Res Inst Biosci & Biotechnol KRIBB, Ctr Dev & Differentiat, Taejon 305806, South Korea

[6] Wonkwang Univ, Dept Pharmacol, Sch Med, Iksan 570749, South Korea

[7] RDA, Natl Acad Agr Sci, Dept Agrofood Resources, Suwon 441853, South Korea

来源：

BMB REPORTS | 2011年 / 44卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

Ganglioside GQ1b; MAP kinase; Mouse embryonic stem cells (mESCs); Neuronal differentiation; ST8 alpha-N-acetyl-neuraminidase alpha-2; 8-sialyltransferase 5 (ST8Sia5); ACTIVATED PROTEIN-KINASES; NEURAL DIFFERENTIATION; PC12; CELLS; EXPRESSION; PROLIFERATION; ERK1/2; GLYCOSPHINGOLIPIDS; GROWTH; GD3;

D O I：

10.5483/BMBRep.2011.44.12.799

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is related to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway. [BMB reports 2011; 44(12): 799-804]

引用

页码：799 / 804

页数：6

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