Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay

被引：20
作者：
Onitsuka, Masayoshi [1 ]
Omasa, Takeshi [1 ]
机构：
[1] Univ Tokushima, Inst Sci & Technol, Tokushima 7708506, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2015年 / 120卷 / 01期
基金：
日本学术振兴会;
关键词：
Glycosylation; Antibody; Chinese hamster ovary cells; Lectin; Chemiluminescent assay; HAMSTER OVARY CELLS; FRONTAL AFFINITY-CHROMATOGRAPHY; OLIGOSACCHARIDE SPECIFICITY; CHO-CELLS; CULTURE;
D O I：
10.1016/j.jbiosc.2014.11.015
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We constructed an assay system for the rapid detection of the glycosylation status of antibodies with a chemiluminescence-based lectin-binding assay, and investigated the glycosylation dynamics during the culture of recombinant Chinese hamster ovary cells. This rapid detection system is applicable to the high-throughput evaluation of therapeutic glycoproteins. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：107 / 110
页数：4
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