Expression and distribution of SP and its NK1 receptor in the brain-gut axis in neonatal maternally separated rat model with visceral hypersensitivity

被引：5
作者：
Teng, W. [1 ]
Chen, H. [2 ]
Guo, F. [2 ]
Du, X. [3 ]
Fu, X. [2 ]
Fang, Y. [4 ]
Zhang, H.
Fang, M. [3 ]
Ding, M. [2 ]
机构：
[1] Zhejiang Univ, Jinhua Hosp, Endoscopy Ctr, Jinhua, Peoples R China
[2] Jinhua Polytech, Dept Med Sci, Jinhua, Peoples R China
[3] Zhejiang Univ, Sch Med, Inst Neurosci, Hangzhou, Zhejiang, Peoples R China
[4] JinHua Ctr Lab Anim, Jinhua, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2016年 / 15卷 / 03期
关键词：
Irritable bowel syndrome; Neonatal maternal separation; Substance P; Neurokinin-1; receptor; Brain-gut axis; Rat; IRRITABLE-BOWEL-SYNDROME; NOXIOUS COLORECTAL DISTENSION; SUBSTANCE-P; COLONIC DISTENSION; STRESS; HYPERALGESIA; NEUROKININ; SYSTEM; GENE; PAIN;
D O I：
10.4238/gmr.15038999
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Neurokinin-1 receptor (NK1R) is a high affinity Substance P(SP) receptor and plays a key role in visceral hypersensitivity in irritable bowel syndrome (IBS). Early life stress is a significant risk factor in IBS. The aim of the present study was to investigate the influence of neonatal maternal separation on the expression and distribution of SP and its receptor along the brain-gut axis in a neonatal maternally separated rat model with visceral hypersensitivity. Male neonatal Sprague-Dawley rats, 2-21-day old, were randomly distributed into maternal separation groups of 3 h daily maternal separation (MS) or non-handling (NH). These rats underwent colorectal balloon distention (CRD) upon reaching adulthood. Immunofluorescence was used to examine the distal colon, lumbosacral spinal cord, and the brainstem to semi-quantitatively determine SP and NK1R expression before and after CRD. The following features were assessed: percentage SP-positive area in colonic muscle layer, the number of NK1R-positive myenteric plexus, SP-positive area and NK1-positivity score in the dorsal horn and the brainstem. Neither of these was altered in the MS and NH groups before or after CRD. These results suggest that the SP system might play little role in the development of visceral hyperalgesia in the neonatal maternal separation rat model.
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页数：12
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共 30 条
[11]   SERT and TPH-1 mRNA expression are reduced in irritable bowel syndrome patients regardless of visceral sensitivity state in large intestine [J].
Kerckhoffs, Angele P. M. ;
ter Linde, Jose J. M. ;
Akkermans, Louis M. A. ;
Samsom, Melvin .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2012, 302 (09) :G1053-G1060
[12]   Alterations in mucosal neuropeptides in patients with irritable bowel syndrome and ulcerative colitis in remission: A role in pain symptom generation? [J].
Keszthelyi, D. ;
Troost, F. J. ;
Jonkers, D. M. ;
Helyes, Z. ;
Hamer, H. M. ;
Ludidi, S. ;
Vanhoutvin, S. ;
Venema, K. ;
Dekker, J. ;
Szolcsanyi, J. ;
Masclee, A. A. .
EUROPEAN JOURNAL OF PAIN, 2013, 17 (09) :1299-1306
[13]   The 5-HT3 receptor antagonist alosetron inhibits the colorectal distention induced depressor response and spinal c-fos expression in the anaesthetised rat [J].
Kozlowski, CM ;
Green, A ;
Grundy, D ;
Boissonade, FM ;
Bountra, C .
GUT, 2000, 46 (04) :474-480
[14]   Identification of the visceral pain pathway activated by noxious colorectal distension in mice [J].
Kyloh, Melinda ;
Nicholas, Sarah ;
Zagorodnyuk, Vladimir P. ;
Brookes, Simon J. ;
Spencer, Nick J. .
FRONTIERS IN NEUROSCIENCE, 2011, 5
[15]   Deficits in visceral pain and hyperalgesia of mice with a disruption of the tachykinin NK1 receptor gene [J].
Laird, JMA ;
Olivar, T ;
Roza, C ;
De Felipe, C ;
Hunt, SP ;
Cervero, F .
NEUROSCIENCE, 2000, 98 (02) :345-352
[16]   Tachykinins and tachykinin receptors in the gut, with special reference to NK2 receptors in human [J].
Lecci, Alessandro ;
Capriati, Angela ;
Altamura, Maria ;
Maggi, Carlo Alberto .
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, 2006, 126 :232-249
[17]   Changes of the neuropeptides content and gene expression in spinal cord and dorsal root ganglion after noxious colorectal distension [J].
Lu, CL ;
Pasricha, PJ ;
Hsieh, JC ;
Lu, RH ;
Lai, CR ;
Wu, LL ;
Chang, FY ;
Lee, SD .
REGULATORY PEPTIDES, 2005, 131 (1-3) :66-73
[18]   Effect of electro-acupuncture on substance P, its receptor and corticotropin-releasing hormone in rats with irritable bowel syndrome [J].
Ma, Xiao-Peng ;
Tan, Lin-Ying ;
Yang, Yun ;
Wu, Huan-Gan ;
Jiang, Bin ;
Liu, Hui-Rong ;
Yang, Ling .
WORLD JOURNAL OF GASTROENTEROLOGY, 2009, 15 (41) :5211-5217
[19]  
Mulè F, 2000, NEUROGASTROENT MOTIL, V12, P459
[20]   Neonatal maternal separation in the rat impacts on the stress responsivity of central corticotropin-releasing factor receptors in adulthood [J].
O'Malley, Dervla ;
Dinan, Timothy G. ;
Cryan, John F. .
PSYCHOPHARMACOLOGY, 2011, 214 (01) :221-229
123