Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO

被引：24

作者：

Psenicka, Martin ^{[2
]}

Dietrich, Grzegorz J. ^{[1
]}

Wojtczak, Mariola ^{[1
]}

Nynca, Joanna ^{[1
]}

Rodina, Marek ^{[2
]}

Linhart, Otomar ^{[2
]}

Cosson, Jacky ^{[3
]}

Ciereszko, Andrzej ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Anim Reprod & Food Res, PL-10747 Olsztyn, Poland

[2] Univ S Bohemia, Res Inst Fish Culture & Hydrobiol, Vodnany 38925, Czech Republic

[3] Univ Paris 06, CNRS, UMR 7009, Observ Villefranche Mer,Marine Stn, F-06230 Villefranche Sur Mer, France

来源：

CRYOBIOLOGY | 2008年 / 56卷 / 03期

关键词：

sturgeon; spermatozoa; acrosome; cryopreservation;

D O I：

10.1016/j.cryobiol.2008.03.006

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this study we describe acrosome staining and motility characteristics of fresh and cryopreserved sterlet (Acipenser ruthenus L.) spermatozoa using soybean trypsin inhibitor-Alexa conjugate fluorescent staining and computer-aided sperm analysis (CASA), respectively. Methanol or dimethylsulfoxide (DMSO) were used as cryoprotectants. After cryopreservation a decline in sperm motility characteristics occurred, but no differential effect between cryoprotectant was observed. Cryopreservation caused a significant increase in the percentage of spermatozoa with acrosome stained by SBTI-Alexa for samples cryopreserved using DMSO compared to methanol. These data suggest that the low usefulness of DMSO for cryopreservation of sturgeon spermatozoa is related to its harmful specific effect towards the acrosome, probably by causing its precocious triggering, much before any egg contact. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：251 / 253

页数：3

共 13 条

[1]

Ciereszko A, 1996, MOL REPROD DEV, V45, P72, DOI 10.1002/(SICI)1098-2795(199609)45:1&lt

[2]

72::AID-MRD10&gt

[3]

3.0.CO

[4]

2-Z

[5] Effect of cryopreservation and theophylline on motility characteristics of lake sturgeon (Acipenser fulvescens) spermatozoa [J].

Ciereszko, A ;

Toth, GP ;

Christ, SA ;

Dabrowski, K .

THERIOGENOLOGY, 1996, 45 (03) :665-672

[6] Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol [J].

Glogowski, J ;

Kolman, R ;

Szczepkowski, M ;

Horváth, A ;

Urbányi, B ;

Sieczynski, P ;

Rzemieniecki, A ;

Domagala, J ;

Demianowicz, W ;

Kowalski, R ;

Ciereszko, A .

AQUACULTURE, 2002, 211 (1-4) :367-373

[7] The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species [J].

Horváth, A ;

Wayman, WR ;

Urbányi, B ;

Ware, KM ;

Dean, JC ;

Tiersch, TR .

AQUACULTURE, 2005, 247 (1-4) :243-251

[8]

HORVATH A, P 6 INT S REPR BIOL, P441

[9] Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L. [J].

Lahnsteiner, F ;

Berger, B ;

Horvath, A ;

Urbanyi, B .

AQUACULTURE RESEARCH, 2004, 35 (06) :519-528

[10]

LEUNG LXP, FISH EVOLUTION SYSTE, P245

← 1 2 →