Recent advances in sortase-catalyzed ligation methodology

被引：126

作者：

Antos, John M. ^{[1
]}

Truttmann, Matthias C. ^{[2
]}

Ploegh, Hidde L. ^{[2
]}

机构：

[1] Western Washington Univ, Dept Chem, 516 High St, Bellingham, WA 98229 USA

[2] Whitehead Inst Biomed Res, Cambridge Ctr 9, Cambridge, MA 02142 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2016年 / 38卷

基金：

瑞士国家科学基金会;

关键词：

STAPHYLOCOCCUS-AUREUS SORTASE; SITE-SPECIFIC MODIFICATION; PROTEIN LIGATION; IMMOBILIZED SORTASE; MEDIATED LIGATIONS; BUTELASE; PURIFICATION; SPECIFICITY; STEP; CYCLIZATION;

D O I：

10.1016/j.sbi.2016.05.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transpeptidation reaction catalyzed by bacterial sortases continues to see increasing use in the construction of novel protein derivatives. In addition to growth in the number of applications that rely on sortase, this field has also seen methodology improvements that enhance reaction performance and scope. In this opinion, we present an overview of key developments in the practice and implementation of sortase-based strategies, including applications relevant to structural biology. Topics include the use of engineered sortases to increase reaction rates, the use of redesigned acyl donors and acceptors to mitigate reaction reversibility, and strategies for expanding the range of substrates that are compatible with a sortase-based approach.

引用

页码：111 / 118

页数：8

共 60 条

[1] Rapid addition of unlabeled silent solubility tags to proteins using a new substrate-fused sortase reagent [J].

Amer, Brendan R. ;

Macdonald, Ramsay ;

Jacobitz, Alex W. ;

Liauw, Brandon ;

Clubb, Robert T. .

JOURNAL OF BIOMOLECULAR NMR, 2016, 64 (03) :197-205

[2] Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity [J].

Antos, John M. ;

Chew, Guo-Liang ;

Guimaraes, Carla P. ;

Yoder, Nicholas C. ;

Grotenbreg, Gijsbert M. ;

Popp, Maximilian Wei-Lin ;

Ploegh, Hidde L. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (31) :10800-+

[3] A Noncanonical Function of Sortase Enables Site-Specific Conjugation of Small Molecules to Lysine Residues in Proteins [J].

Bellucci, Joseph J. ;

Bhattacharyya, Jayanta ;

Chilkoti, Ashutosh .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2015, 54 (02) :441-445

[4] Three-in-One Chromatography-Free Purification, Tag Removal, and Site-Specific Modification of Recombinant Fusion Proteins Using SortaseA and Elastin-like Polypeptides [J].

Bellucci, Joseph J. ;

Amiram, Miriam ;

Bhattacharyya, Jayanta ;

McCafferty, Dewey ;

Chilkoti, Ashutosh .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (13) :3703-3708

[5] A general strategy for the evolution of bond-forming enzymes using yeast display [J].

Chen, Irwin ;

Dorr, Brent M. ;

Liu, David R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (28) :11399-11404

[6] Sortase A-mediated multi-functionalization of protein nanoparticles [J].

Chen, Qi ;

Sun, Qing ;

Molino, Nicholas M. ;

Wang, Szu-Wen ;

Boder, Eric T. ;

Chen, Wilfred .

CHEMICAL COMMUNICATIONS, 2015, 51 (60) :12107-12110

[7] Reprogramming the specificity of sortase enzymes [J].

Dorr, Brent M. ;

Ham, Hyun Ok ;

An, Chihui ;

Chaikof, Elliot L. ;

Liu, David R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (37) :13343-13348

[8] Structurally Defined aMHC-II Nanobody-Drug Conjugates: A Therapeutic and Imaging System for B-Cell Lymphoma [J].

Fang, Tao ;

Duarte, Joao N. ;

Ling, Jingjing ;

Li, Zeyang ;

Guzman, Jonathan S. ;

Ploegh, Hidde L. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 55 (07) :2416-2420

[9] Efficient segmental isotope labeling of multi-domain proteins using Sortase A [J].

Freiburger, Lee ;

Sonntag, Miriam ;

Hennig, Janosch ;

Li, Jian ;

Zou, Peijian ;

Sattler, Michael .

JOURNAL OF BIOMOLECULAR NMR, 2015, 63 (01) :1-8

[10]

Gianella P, 2016, BIOTECHNOL BIOENG

← 1 2 3 4 5 6 →