Fabrication of amperometric biosensors by entrapment of enzymes in functionalized polypyrrole films

被引：14
作者：
Cosnier, S [1 ]
机构：
[1] Univ Grenoble 1, UMR CNRS 5630, Lab Electrochim Organ & Photochim Redox, F-38041 Grenoble 9, France
来源：
CANADIAN JOURNAL OF CHEMICAL ENGINEERING | 1998年 / 76卷 / 06期
关键词：
biosensors; electropolymerization; enzyme electrodes; polypyrrole; wired enzymes;
D O I：
10.1002/cjce.5450760605
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
The concept and potentialities of an original electrochemical procedure of enzyme immobilization are described. This two-step method consists of the adsorption of an aqueous amphiphilic pyrrole monomer-enzyme mixture on an electrode surface followed by the electropolymerization of the adsorbed monomers. This procedure is compared with the conventional electrochemical methods of biosensor construction. Several bienzyme electrodes devoted to the improvement of the biosensor selectivity or to the amplification of the biosensor sensitivity are described. In addition, examples of organic phase enzyme electrode and the electrical wiring of immobilized enzymes are presented.
引用
收藏
页码：1000 / 1007
页数：8
相关论文
共 51 条
[1]  
BARLETT PN, 1987, J CHEM SOC CHEM COMM, P1603
[2]   ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES .1. THEORY [J].
BARTLETT, PN ;
WHITAKER, RG .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 224 (1-2) :27-35
[3]   ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES .5. MICROELECTRODES FOR THE DETECTION OF GLUCOSE BASED ON GLUCOSE-OXIDASE IMMOBILIZED IN A POLY(PHENOL) FILM [J].
BARTLETT, PN ;
CARUANA, DJ .
ANALYST, 1992, 117 (08) :1287-1292
[4]  
BARTLETT PN, 1993, J ELECTROANAL CHEM, V363, P1
[5]   NEW ENZYME SENSOR FOR PHENOL DETERMINATION IN NONAQUEOUS AND AQUEOUS-MEDIUM [J].
CAMPANELLA, L ;
SAMMARTINO, MP ;
TOMASSETTI, M .
SENSORS AND ACTUATORS B-CHEMICAL, 1992, 7 (1-3) :383-388
[6]   HIGH SURFACE-AREA CATALYSTS FOR H-2 REDUCTION OF AN ENZYME - REDUCTION OF NAD+ TO NADH [J].
CHAO, S ;
WRIGHTON, MS .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (19) :5886-5888
[7]   POLY(AMPHIPHILIC PYRROLE)-PPO ELECTRODES FOR ORGANIC-PHASE ENZYMATIC ASSAY [J].
COCHEGUERENTE, L ;
COSNIER, S ;
INNOCENT, C .
ANALYTICAL LETTERS, 1995, 28 (06) :1005-1016
[8]   ELECTROPOLYMERIZATION OF CATIONIC AMPHIPHILIC PYRROLE DERIVATIVES ON ELECTRODES - EVIDENCE FOR ENVIRONMENTAL-EFFECTS ON REDOX POTENTIALS OF TRAPPED ANIONS [J].
COCHEGUERENTE, L ;
DERONZIER, A ;
GALLAND, B ;
MOUTET, JC ;
LABBE, P ;
REVERDY, G ;
CHEVALIER, Y ;
AMHRAR, J .
LANGMUIR, 1994, 10 (02) :602-610
[9]   DEVELOPMENT OF AMPEROMETRIC BIOSENSORS BASED ON THE IMMOBILIZATION OF ENZYMES IN POLYMER-FILMS ELECTROGENERATED FROM A SERIES OF AMPHIPHILIC PYRROLE DERIVATIVES [J].
COCHEGUERENTE, L ;
COSNIER, S ;
INNOCENT, C ;
MAILLEY, P .
ANALYTICA CHIMICA ACTA, 1995, 311 (01) :23-30
[10]   ELECTROCHEMICAL IMMOBILIZATION OF GLUCOSE-OXIDASE IN POLY(AMPHIPHILIC PYRROLE) FILMS AND ITS APPLICATION TO THE PREPARATION OF AN AMPEROMETRIC GLUCOSE SENSOR [J].
COCHEGUERENTE, L ;
DERONZIER, A ;
MAILLEY, P ;
MOUTET, JC .
ANALYTICA CHIMICA ACTA, 1994, 289 (02) :143-153
123456