T-Cell Assay after COVID-19 Vaccination Could Be a Useful Tool? A Pilot Study on Interferon-Gamma Release Assay in Healthcare Workers

被引：8

作者：

Seraceni, Silva ^{[1
]}

Zocca, Elena ^{[1
]}

Cervone, Tamara Emanuela ^{[1
]}

Tomassetti, Flaminia ^{[2
,3
]}

Polidori, Isabella ^{[3
]}

Valisi, Massimiliano ^{[4
]}

Broccolo, Francesco ^{[4
,5
]}

Calugi, Graziella ^{[3
]}

Bernardini, Sergio ^{[2
,6
]}

Pieri, Massimo ^{[2
,6
]}

机构：

[1] Lifebrain RDI Srl, I-35010 Padua, Italy

[2] Univ Roma Tor Vergata, Dept Expt Med, I-00133 Rome, Italy

[3] Lifebrain Srl, I-00133 Rome, Italy

[4] Cerba HealthCare Italia, I-20139 Milan, Italy

[5] Univ Milano Bicocca, Dept Med & Surg, I-20900 Monza, Italy

[6] Tor Vergata Univ Hosp, Dept Lab Med, I-00100 Rome, Italy

来源：

DISEASES | 2022年 / 10卷 / 03期

关键词：

adaptive responses; COVID-19; IGRA test; INF-gamma; T-cells; vaccination; SARS-COV-2;

D O I：

10.3390/diseases10030049

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background: SARS-CoV-2 T-cells are crucial for long-term protection against reinfection. The aim was to demonstrate the Interferon-gamma Release Assay (IGRA) test could be useful for vaccination monitoring. Methods: In a prospective cohort of 98 vaccinated healthcare workers for SARS-CoV-2, we selected 23 people in low-antibodies (Group 1, N = 8), high-antibodies (Group 2, N = 9), and negative control groups (Group 3, N = 6). SARS-CoV-2-specific humoral and cellular responses were analyzed at 8 months after two doses of Pfizer BioNTech, evaluating anti-RBD (Receptor Binding Domain) and RBD-ACE2 (Angiotensin Converting Enzyme-2) blocking antibodies in sera through a Chemiluminescence Immunoassay (CLIA) and T-cells through the IGRA test in heparinized plasma. Moreover, lymphocyte subtyping was executed by a flow cytometer. Statistical analysis was performed. Results: The data confirmed that RBD and RBD-ACE2 blocking ACE2 antibody levels of Group 1 were significantly lower than Group 2; p < 0.001. However, T-cells showed no significant difference between Group 1 and Group 2. Conclusions: This work suggests the need for new strategies for booster doses administration.

引用

页数：8

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