Lessons from GNE-deficient embryonic stem cells: sialic acid biosynthesis is involved in proliferation and gene expression

被引：37

作者：

Weidemann, Wenke ^{[1
]}

Klukas, Christian ^{[2
]}

Klein, Andreas ^{[3
]}

Simm, Andreas ^{[4
]}

Schreiber, Falk ^{[2
,5
]}

Horstkorte, Ruediger ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Inst Physiol Chem, D-06114 Halle, Germany

[2] Leibniz Inst Plant Genet & Crop Plant Res IPK Gat, D-06466 Gatersleben, Germany

[3] Charite, Inst Mol Biol & Bioinformat, D-14195 Berlin, Germany

[4] Dept Cardiothorac Surg, D-06120 Halle, Germany

[5] Univ Halle Wittenberg, Inst Comp Sci, D-06120 Halle, Germany

来源：

GLYCOBIOLOGY | 2010年 / 20卷 / 01期

关键词：

cell differentiation; N-acetylmannosamine; N-acetylmannosamine kinase; UDP-N-acetylglucosamine-2-epimerase; N-ACETYLNEURAMINIC ACID; 2-EPIMERASE/N-ACETYLMANNOSAMINE KINASE GNE; BIFUNCTIONAL ENZYME CATALYZES; RING FINGER GENE; 1ST; STEPS; OPITZ-SYNDROME; RAT-LIVER; BIOLOGICAL FUNCTIONS; GROWTH-FACTOR; MOUSE;

D O I：

10.1093/glycob/cwp153

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sialic acids are widely expressed as terminal carbohydrates on glycoconjugates of eukaryotic cells. They are involved in a variety of cellular functions, such as cell adhesion or signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyzes the first two steps of sialic acid biosynthesis in the cytosol. Inactivation of GNE causes early embryonic lethality. In this study, we analyzed wild-type and GNE-deficient embryonic stem cells from mice. We found for the first time that proliferation is directly correlated with GNE-expression and the cellular sialic acid concentration. Furthermore, we identified growth-related genes that are differentially expressed in GNE-deficient embryonic stem cells compared to wildtype embryonic stem cells.

引用

页码：107 / 117

页数：11

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