Analysis of Myeloid Cells in Mouse Tissues with Flow

被引：59
作者：
Liu, Zhaoyuan [1 ]
Gu, Yaqi [1 ]
Shin, Amanda [1 ]
Zhang, Shuangyan [1 ]
Ginhoux, Florent [1 ,2 ,3 ]
机构：
[1] Shanghai Jiao Tong Univ, Shanghai Inst Immunol, Dept Immunol & Microbiol, Sch Med, Shanghai 200025, Peoples R China
[2] Agcy Sci Technol & Res, Singapore Immunol Network, Singapore 138648, Singapore
[3] SingHealth Duke NUS Acad Med Ctr, Translat Immunol Inst, Singapore, Singapore
来源：
STAR PROTOCOLS | 2020年 / 1卷 / 01期
基金：
新加坡国家研究基金会; 中国国家自然科学基金;
关键词：
ADULT MICROGLIA;
D O I：
10.1016/j.xpro.2020.100029
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019).
引用
收藏
页数：17
相关论文
共 8 条
  • [1] Constant replenishment from circulating monocytes maintains the macrophage pool in the intestine of adult mice
    Bain, Calum C.
    Bravo-Blas, Alberto
    Scott, Charlotte L.
    Perdiguero, Elisa Gomez
    Geissmann, Frederic
    Henri, Sandrine
    Malissen, Bernard
    Osborne, Lisa C.
    Artis, David
    Mowat, Allan Mci
    [J]. NATURE IMMUNOLOGY, 2014, 15 (10) : 929 - U236
  • [2] Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches
    Chakarov, Svetoslav
    Lim, Hwee Ying
    Tan, Leonard
    Lim, Sheau Yng
    See, Peter
    Lum, Josephine
    Zhang, Xiao-Meng
    Foo, Shihui
    Nakamizo, Satoshi
    Duan, Kaibo
    Kong, Wan Ting
    Gentek, Rebecca
    Balachander, Akhila
    Carbajo, Daniel
    Bleriot, Camille
    Malleret, Benoit
    Tam, John Kit Chung
    Baig, Sonia
    Shabeer, Muhammad
    Toh, Sue-Anne Ee Shiow
    Schlitzer, Andreas
    Larbi, Anis
    Marichal, Thomas
    Malissen, Bernard
    Chen, Jinmiao
    Poidinger, Michael
    Kabashima, Kenji
    Bajenoff, Marc
    Ng, Lai Guan
    Angeli, Veronique
    Ginhoux, Florent
    [J]. SCIENCE, 2019, 363 (6432) : 1190 - +
  • [3] Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
    Cossarizza, Andrea
    Chang, Hyun-Dong
    Radbruch, Andreas
    Acs, Andreas
    Adam, Dieter
    Adam-Klages, Sabine
    Agace, William W.
    Aghaeepour, Nima
    Akdis, Mubeccel
    Allez, Matthieu
    Almeida, Larissa Nogueira
    Alvisi, Giorgia
    Anderson, Graham
    Andrae, Immanuel
    Annunziato, Francesco
    Anselmo, Achille
    Bacher, Petra
    Baldari, Cosima T.
    Bari, Sudipto
    Barnaba, Vincenzo
    Barros-Martins, Joana
    Battistini, Luca
    Bauer, Wolfgang
    Baumgart, Sabine
    Baumgarth, Nicole
    Baumjohann, Dirk
    Baying, Bianka
    Bebawy, Mary
    Becher, Burkhard
    Beisker, Wolfgang
    Benes, Vladimir
    Beyaert, Rudi
    Blanco, Alfonso
    Boardman, Dominic A.
    Bogdan, Christian
    Borger, Jessica G.
    Borsellino, Giovanna
    Boulais, Philip E.
    Bradford, Jolene A.
    Brenner, Dirk
    Brinkman, Ryan R.
    Brooks, Anna E. S.
    Busch, Dirk H.
    Buescher, Martin
    Bushnell, Timothy P.
    Calzetti, Federica
    Cameron, Garth
    Cammarata, Ilenia
    Cao, Xuetao
    Cardell, Susanna L.
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2019, 49 (10) : 1457 - 1973
  • [4] Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages
    Ginhoux, Florent
    Greter, Melanie
    Leboeuf, Marylene
    Nandi, Sayan
    See, Peter
    Gokhan, Solen
    Mehler, Mark F.
    Conway, Simon J.
    Ng, Lai Guan
    Stanley, E. Richard
    Samokhvalov, Igor M.
    Merad, Miriam
    [J]. SCIENCE, 2010, 330 (6005) : 841 - 845
  • [5] Fate Mapping via Ms4a3-Expression History Traces Monocyte-Derived Cells
    Liu, Zhaoyuan
    Gu, Yaqi
    Chakarov, Svetoslav
    Bleriot, Camille
    Kwok, Immanuel
    Chen, Xin
    Shin, Amanda
    Huang, Weijie
    Dress, Regine J.
    Dutertre, Charles-Antoine
    Schlitzer, Andreas
    Chen, Jinmiao
    Ng, Lai Guan
    Wang, Honglin
    Liu, Zhiduo
    Su, Bing
    Ginhoux, Florent
    [J]. CELL, 2019, 178 (06) : 1509 - +
  • [6] Tissue-resident macrophages in the intestine are long lived and defined by Tim-4 and CD4 expression
    Shaw, Tovah N.
    Houston, Stephanie A.
    Wemyss, Kelly
    Bridgeman, Hayley M.
    Barbera, Thomas A.
    Zangerle-Murray, Tamsin
    Strangward, Patrick
    Ridley, Amanda J. L.
    Wang, Ping
    Tamoutounour, Samira
    Allen, Judith E.
    Konkel, Joanne E.
    Grainger, John R.
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 2018, 215 (06) : 1507 - 1518
  • [7] Origins and Functional Specialization of Macrophages and of Conventional and Monocyte-Derived Dendritic Cells in Mouse Skin
    Tamoutounour, Samira
    Guilliams, Martin
    Sanchis, Frederic Montanana
    Liu, Hong
    Terhorst, Dorothea
    Malosse, Camille
    Pollet, Emeline
    Ardouin, Laurence
    Luche, Herve
    Sanchez, Cindy
    Dalod, Marc
    Malissen, Bernard
    Henri, Sandrine
    [J]. IMMUNITY, 2013, 39 (05) : 925 - 938
  • [8] Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner
    Thion, Morgane Sonia
    Low, Donovan
    Silvin, Aymeric
    Chen, Jinmiao
    Grisel, Pauline
    Schulte-Schrepping, Jonas
    Blecher, Ronnie
    Ulas, Thomas
    Squarzoni, Paola
    Hoeffel, Guillaume
    Coulpier, Fanny
    Siopi, Eleni
    David, Friederike Sophie
    Scholz, Claus
    Shihui, Foo
    Lum, Josephine
    Amoyo, Arlaine Anne
    Larbi, Anis
    Poidinger, Michael
    Buttgereit, Anne
    Lledo, Pierre-Marie
    Greter, Melanie
    Chan, Jerry Kok Yen
    Amit, Ido
    Beyer, Marc
    Schultze, Joachim Ludwig
    Schlitzer, Andreas
    Pettersson, Sven
    Ginhoux, Florent
    Garel, Sonia
    [J]. CELL, 2018, 172 (03) : 500 - +
1