Cytogenetic analysis of two related Deltochilum (Coleoptera, Scarabaeidae) species: Diploid number reduction, extensive heterochromatin addition and differentiation

被引：13
作者：
Cabral-de-Mello, D. C. [1 ,2 ]
Moura, R. C. [2 ]
Carvalho, R. [3 ]
Souza, M. J. [1 ]
机构：
[1] Univ Fed Pernambuco, Ctr Ciencias Biol, Dept Genet, BR-50732970 Recife, PE, Brazil
[2] Univ Pernambuco, Inst Ciencias Biol, Dept Biol, Recife, PE, Brazil
[3] Univ Fed Rural Pernambuco, Dept Biol Genet, Recife, PE, Brazil
来源：
MICRON | 2010年 / 41卷 / 02期
关键词：
Chromosome rearrangement; Heterochromatin amplification; Karyotype; Meiosis; rDNA; Y-AUTOSOME TRANSLOCATION; KARYOTYPE DIFFERENTIATION; CONSTITUTIVE HETEROCHROMATIN; NUCLEOTIDE-SEQUENCE; ORGANIZER REGIONS; SATELLITE DNA; PATTERNS; BEETLE; SCARABAEOIDEA; CHROMOSOMES;
D O I：
10.1016/j.micron.2009.10.005
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Male mitotic and meiotic chromosomes of two species of the genus Deltochilum (Scarabaeidae) were analyzed through conventional staining, C-banding, base-specific fluorochromes, silver nitrate staining (AgNO3) and FISH (45S rDNA). The two species possessed karyotypes with 2n = 14, neo-XY and meta-submetacentric chromosomes. The analysis of constitutive heterochromatin (CH) revealed mainly diphasic chromosomes in the two species, showing heterochromatic long arms. Silver nitrate staining labeled the blocks corresponding to CH in D. (Deltohyboma) aff morbillosum while in D. (Deltohyboma) calcaratum, AgNO3 staining revealed only the CH blocks of the diphasic autosomes. The fluorochrome staining revealed in D. (D.) calcaratum the diphasic autosomes and the sex chromosomes with CMA(3)(+) blocks, and in D. (D.) aff morbillosum, the GC-rich sequences were restricted to the terminal regions of the long arms of the pairs 1 and 2 and the X. The FISH revealed 45S rDNA sites in two autosomic pairs and in the X chromosome. The analyses performed allowed for the identification of cytogenetic markers and the discussion of possible chromosome rearrangements that have been involved in the karyotypic differentiation of these species mainly related to the repetitive genome. (c) 2009 Elsevier Ltd. All rights reserved.
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页码：112 / 117
页数：6
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