Influence of amyloid-β peptides with different lengths and amino acid sequences on the lateral diffusion of lipids in model membranes

被引:16
作者
Buchsteiner, Alexandra [1 ]
Hauss, Thomas [1 ]
Dencher, Norbert A. [2 ]
机构
[1] Helmholtz Zentrum Berlin Mat & Energie, D-14109 Berlin, Germany
[2] Tech Univ Darmstadt, Dept Chem, D-64287 Darmstadt, Germany
关键词
ALZHEIMERS-DISEASE; NMR;
D O I
10.1039/c1sm06823g
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We have investigated the influence of two neurotoxic Alzheimer's disease peptide analogues with different lengths and amino acid compositions, amyloid-beta(25-35) and amyloid-beta(22-40), on the dynamics of phospholipid membranes by means of quasi-elastic neutron scattering in the picosecond time domain. Samples of pure phospholipids (DMPC/DMPS) and samples with embedded amyloid-beta peptides have been compared. The peptide concentration was 3 mol% for both amyloid-beta peptides. The sample temperature was set to 320 K, where the samples are in the liquid crystalline phase, which is the physiologically relevant phase of biological membranes. The data have been analyzed with a purely phenomenological model, which combines two different motions with different time constants. These two motions have been assigned to a long-range translational diffusion and a spatially restricted (localized) diffusion. The different lengths of the peptides leads to different positions and orientations inside the membrane bilayers. Thus, different influences on the dynamics are also expected. Both amyloid-beta peptides significantly affected the ps-dynamics of oriented lipid membranes. Mainly, they accelerated the long-range translational diffusion even though the peptide concentration was quite low. This finding is of relevance for all kinds of protein-protein interactions, which are strongly influenced by the lateral diffusion, such as signal transduction cascades and energy transfer. This influence might be involved in the pathology of Alzheimer's disease as well as being a clue for early diagnosis and in therapy.
引用
收藏
页码:424 / 429
页数:6
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