Classic Maximum Entropy Recovery of the Average Joint Distribution of Apparent FRET Efficiency and Fluorescence Photons for Single-Molecule Burst Measurements

被引：7

作者：

DeVore, Matthew S. ^{[1
]}

Gull, Stephen F. ^{[2
]}

Johnson, Carey K. ^{[1
]}

机构：

[1] Univ Kansas, Dept Chem, Lawrence, KS 66045 USA

[2] Univ Cambridge, Dept Phys, Astrophys Grp, Cambridge CB3 0HE, England

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2012年 / 116卷 / 13期

基金：

美国国家科学基金会;

关键词：

RESONANCE ENERGY-TRANSFER; INTENSITY; DYNAMICS; HISTOGRAMS; LIFETIME; STATES;

D O I：

10.1021/jp209861u

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We describe a method for analysis of single-molecule Forster resonance energy transfer (FRET) burst measurements using classic maximum entropy. Classic maximum entropy determines the Bayesian inference for the joint probability describing the total fluorescence photons and the apparent FRET efficiency. The method was tested with simulated data and then with DNA labeled with fluorescent dyes. The most probable joint distribution can be marginalized to obtain both the overall distribution of fluorescence photons and the apparent FRET efficiency distribution. This method proves to be ideal for determining the distance distribution of FRET-labeled biomolecules, and it successfully predicts the shape of the recovered distributions.

引用

页码：4006 / 4015

页数：10

共 46 条

[1]

[Anonymous], 1990, Maximum Entropy and Bayesian Methods

[2] Separating structural heterogeneities from stochastic variations in fluorescence resonance energy transfer distributions via photon distribution analysis [J].