Temperature-pressure correlations of cryoprotective additives for the design of constant volume cryopreservation protocols

被引:2
作者
Beschea, George-Andrei [1 ]
Tabacaru, Maria-Bianca [1 ]
Campean, Stefan-Ioan [1 ]
Serban, Alexandru [2 ]
Vutoiu, Beatrice-Georgiana [1 ]
Stefanescu, Mariana-Florentina [2 ]
Dobrovicescu, Alexandru [2 ]
Nastase, Gabriel [1 ,2 ]
机构
[1] Transilvania Univ Brasov, Fac Civil Engn, Dept Bldg Serv, Brasov, Romania
[2] Univ Politehn Bucuresti, Fac Mech Engn & Mechatron, Thermal & Refrigerat Equipment Dept, Thermotech,Engines, Bucharest, Romania
关键词
Cryoprotective additives; Isochoric; Temperature -Pressure correlations; Dimethyl sulfoxide; Trehalose; Ethylene glycol; Diethylene glycol; MOUSE EMBRYOS; RAT HEARTS; PRESERVATION; SYSTEM; CELLS; ORGAN;
D O I
10.1016/j.cryobiol.2022.08.001
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the recent years, the use of constant volume (isochoric) cryopreservation, in medicine and biotechnology has captured more attention from the research community and now there is an increasing interest in the use of this new technology. It has been established that the thermodynamics of isochoric freezing is different from that of isobaric (constant pressure) freezing. This study provides researchers in the field experimental results for various compositions of cryoprotectants commonly used in isobaric cryopreservation, in terms of temperature-pressure -molar concentration correlation. It also reveals experimental isochoric thermodynamic data for the following cryoprotectants, commonly used in isobaric cryopreservation: dimethyl sulfoxide, trehalose, ethylene glycol and diethylene glycol. Currently, the data on the pressure-temperature correlation in an isochoric system of cryo-protectants used in isobaric cryopreservation is not available. Our new experimental results indicate that the studied concentrations for each of the CPAs, lower and expands the range of temperatures in which cryopres-ervation by isochoric freezing can be safely practiced. We consider that these experiments will aid researchers developing new isochoric cryopreservation protocols.
引用
收藏
页码:42 / 50
页数:9
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