Development of a sensitive and fast UHPLC-MS/MS method for determination of clopidogrel, clopidogrel acid and clopidogrel active metabolite H4 in human plasma

被引：9

作者：

Hua, Wenyi ^{[1
]}

Lesslie, Michael ^{[1
]}

Hoffman, Brian T. ^{[1
]}

Binns, Christopher ^{[1
]}

Mulvana, Daniel ^{[1
]}

机构：

[1] Quintiles, Bioanalyt & ADME Labs, Ithaca, NY 14850 USA

来源：

BIOANALYSIS | 2015年 / 7卷 / 12期

关键词：

THIOL METABOLITE; QUANTITATIVE-DETERMINATION; SAMPLES;

D O I：

10.4155/bio.15.82

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Quantitation of CAM H4 in biological matrix has been a challenge due to low concentrations, instability and the existence of four CAM diastereomers. Historically, either inactive clopidogrel acid or CAM diastereomers without separation were measured for exposure and PK parameters. Results: This study presents a sensitive and fast UHPLC-MS/MS method for simultaneous quantitation of clopidogrel, clopidogrel acid and active metabolite H4 in human plasma. The method demonstrated the separation of H4 from other isomers and yet retained clopidogrel acid. Matrix stabilities, accuracy and precision, recovery and matrix effect were evaluated during development. Conclusion: With LLOQ of 0.05 ng/ml for clopidogrel and H4, and 5.5 min LC gradient, it is the most sensitive and fastest method to our knowledge.

引用

页码：1471 / 1482

页数：12

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