Isolation and identification of actinomycetes from a compost-amended soil with potential as biocontrol agents

被引:55
作者
Cuesta, Gonzalo [2 ]
Garcia-de-la-Fuente, Rosana [1 ]
Abad, Manuel [1 ]
Fornes, Fernando [1 ]
机构
[1] Univ Politecn Valencia, Inst Agroforestal Mediterraneo, E-46071 Valencia, Spain
[2] Univ Politecn Valencia, Dept Biotecnol, E-46071 Valencia, Spain
关键词
Streptomyces; Lechevalieria; Phytopathogenic fungi; Composted two-phase olive mill waste; FUSARIUM-WILT; SP NOV; STREPTOMYCES; ANTIBIOTICS;
D O I
10.1016/j.jenvman.2010.11.023
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The search for new biocontrol strategies to inhibit the growth of phytopathogenic microorganisms has become widely widespread due to environmental concerns. Among actinomycetes, Streptomyces species have been extensively studied since they have been recognized as important sources of antibiotics. Actinomycete strains were isolated from a calcareous soil, 2 two-phase olive mill waste ('alperujo') composts, and the compost-amended soil by using selective media, and they were then co-cultured with 5 phytopathogenic fungi and 1 bacterium to perform an in vitro antagonism assay. Forty-nine actinomycete strains were isolated, 12 of them showing a great antagonistic activity towards the phytopathogenic microorganisms tested. Isolated strains were identified by 165 rDNA sequence analysis and phenotypic procedures. Eleven isolates concerned the genus Streptomyces and 1 actinomycete with chitinolytic activity belonged to the genus Lechevalieria. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:S280 / S284
页数:5
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