Isolation and identification of actinomycetes from a compost-amended soil with potential as biocontrol agents

被引：56

作者：

Cuesta, Gonzalo ^{[2
]}

Garcia-de-la-Fuente, Rosana ^{[1
]}

Abad, Manuel ^{[1
]}

Fornes, Fernando ^{[1
]}

机构：

[1] Univ Politecn Valencia, Inst Agroforestal Mediterraneo, E-46071 Valencia, Spain

[2] Univ Politecn Valencia, Dept Biotecnol, E-46071 Valencia, Spain

来源：

JOURNAL OF ENVIRONMENTAL MANAGEMENT | 2012年 / 95卷

关键词：

Streptomyces; Lechevalieria; Phytopathogenic fungi; Composted two-phase olive mill waste; FUSARIUM-WILT; SP NOV; STREPTOMYCES; ANTIBIOTICS;

D O I：

10.1016/j.jenvman.2010.11.023

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The search for new biocontrol strategies to inhibit the growth of phytopathogenic microorganisms has become widely widespread due to environmental concerns. Among actinomycetes, Streptomyces species have been extensively studied since they have been recognized as important sources of antibiotics. Actinomycete strains were isolated from a calcareous soil, 2 two-phase olive mill waste ('alperujo') composts, and the compost-amended soil by using selective media, and they were then co-cultured with 5 phytopathogenic fungi and 1 bacterium to perform an in vitro antagonism assay. Forty-nine actinomycete strains were isolated, 12 of them showing a great antagonistic activity towards the phytopathogenic microorganisms tested. Isolated strains were identified by 165 rDNA sequence analysis and phenotypic procedures. Eleven isolates concerned the genus Streptomyces and 1 actinomycete with chitinolytic activity belonged to the genus Lechevalieria. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：S280 / S284

页数：5

共 25 条

[1] The taxonomy of Streptomyces and related genera [J].

Anderson, AS ;

Wellington, EMH .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :797-814

[2]

Castillo UF, 2002, MICROBIOL-SGM, V148, P2675, DOI 10.1099/00221287-148-9-2675

[3]

Chun J., 1995, Ph.D. Thesis

[4]

FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x

[5]

Felsenstein Joseph, 1993, {PHYLIP}: phylogenetic inference package

[6] Evaluation of Streptomyces sp strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets [J].

Getha, K ;

Vikineswary, S ;

Wong, WH ;

Seki, T ;

Ward, A ;

Goodfellow, M .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2005, 32 (01) :24-32

[7] Chitinolytic actinomycetes from a Brazilian tropical soil active against phytopathogenic fungi [J].

Gomes, RC ;

Semêdo, LTAS ;

Soares, RMA ;

Alviano, CS ;

Linhares, LF ;

Coelho, RRR .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2000, 16 (01) :109-110

[8] Biocontrol within the context of soil microbial communities: A substrate-dependent phenomenon [J].

Hoitink, HAJ ;

Boehm, MJ .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 1999, 37 :427-446

[9] Isolation and in vivo and in vitro antifungal activity of phenylacetic acid and sodium phenylacetate from Streptomyces humidus [J].

Hwang, BK ;

Lim, SW ;

Kim, BS ;

Lee, JY ;

Moon, SS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (08) :3739-3745

[10]

JUKES T H, 1969, P21

← 1 2 3 →