Analysis of protein digests by transmission-mode desorption electrospray ionization mass spectrometry with ultraviolet photodissociation

被引：7

作者：

Shaw, Jared B. ^{[1
]}

Brodbelt, Jennifer S. ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Chem & Biochem, Austin, TX 78712 USA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 2011年 / 308卷 / 2-3期

关键词：

Photodissociation; Desorption electrospray ionization; Proteomics; Peptide sequencing; ASSISTED-LASER-DESORPTION/IONIZATION; AMBIENT CONDITIONS; HPTLC/DESI-MS; MALDI-MS; 157; NM; PEPTIDE; IDENTIFICATION; PROTEOMICS; VERSATILE;

D O I：

10.1016/j.ijms.2011.08.030

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Transmission mode desorption electrospray ionization is coupled with ultraviolet photodissociation at 193 nm for the analysis of protein digests. SEQUEST is utilized for data interpretation and database searches. Comparative results are presented for transmission mode desorption electrospray ionization (TM-DESI)-MS/collision induced dissociation (CID) and TM-DESI-MS/ultraviolet photodissociation (UVPD) analyses of five proteolyzed model proteins ranging in molecular weight from 8.5 kDa (ubiquitin) to 66 kDa (bovine serum albumin, BSA). In some cases TM-DESI/UVPD yielded greater confidence in database correlation scores for peptides and comparable protein identification compared to TM-DESI-MS/CID due to the production of an extensive array of sequence ions and the ability to detect low m/z terminal sequence ions and immonium ions. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：203 / 208

页数：6

共 39 条

[1] Direct high-resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry [J].

Bereman, Michael S. ;

Nyadong, Leonard ;

Fernandez, Facundo M. ;

Muddiman, David C. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2006, 20 (22) :3409-3411

[2] The Influence of Material and Mesh Characteristics on Transmission Mode Desorption Electrospray Ionization [J].

Chipuk, Joseph E. ;

Brodbelt, Jennifer S. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2009, 20 (04) :584-592

[3] Transmission Mode Desorption Electrospray Ionization [J].

Chipuk, Joseph E. ;

Brodbelt, Jennifer S. .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2008, 19 (11) :1612-1620

[4] Versatile new ion source for the analysis of materials in open air under ambient conditions [J].

Cody, RB ;

Laramée, JA ;

Durst, HD .

ANALYTICAL CHEMISTRY, 2005, 77 (08) :2297-2302

[5] Introduction to computational proteomics [J].

Colinge, Jacques ;

Bennett, Keiryn L. .

PLOS COMPUTATIONAL BIOLOGY, 2007, 3 (07) :1151-1160

[6] The nature of collision-induced dissociation processes of doubly protonated peptides: comparative study for the future use of matrix-assisted laser desorption/ionization on a hybrid quadrupole time-of-flight mass spectrometer in proteomics [J].

Cramer, R ;

Corless, S .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (22) :2058-2066

[7] Chromogenic cross-linker for the characterization of protein structure by infrared multiphoton dissociation mass spectrometry [J].

Gardner, Myles W. ;

Vasicek, Lisa A. ;

Shabbir, Shagufta ;

Anslyn, Eric V. ;

Brodbelt, Jennifer S. .

ANALYTICAL CHEMISTRY, 2008, 80 (13) :4807-4819

[8]

Gevaert K, 2001, ELECTROPHORESIS, V22, P1645, DOI 10.1002/1522-2683(200105)22:9<1645::AID-ELPS1645>3.0.CO

[9]

2-Z

[10]

Gevaert K, 2000, ELECTROPHORESIS, V21, P1145, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1145::AID-ELPS1145>3.3.CO

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