Oriented Nanoarchitectonics of Bacteriorhodopsin for Enhancing ATP Generation in a FoF1-ATPase-Based Assembly System

被引：44

作者：

Li, Zibo ^{[1
]}

Xu, Xia ^{[2
,4
]}

Yu, Fanchen ^{[2
,4
]}

Fei, Jinbo ^{[2
,4
]}

Li, Qiang ^{[1
]}

Dong, Mingdong ^{[3
]}

Li, Junbai ^{[2
,4
]}

机构：

[1] Shandong Univ, Key Lab Colloid & Interface Chem, Minist Educ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China

[2] Chinese Acad Sci, Beijing Natl Lab Mol Sci BNLMS, CAS Key Lab Colloid Interface & Chem Thermodynam, Inst Chem, Beijing 100190, Peoples R China

[3] Aarhus Univ, Interdisciplinary Nanosci Ctr iNANO, DK-8000 Aarhus C, Denmark

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2022年 / 61卷 / 16期

基金：

中国博士后科学基金; 中国国家自然科学基金; 欧盟地平线“2020”;

关键词：

energy conversion; proton gradient; ATP production; co-assembly; artificial biomimetic system; MEMBRANE-PROTEIN; RECONSTITUTION; MICROCAPSULES; SYNTHASE; POLYMER;

D O I：

10.1002/anie.202116220

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Energy conversion plays an important role in the metabolism of photosynthetic organisms. Improving energy transformation by promoting a proton gradient has been a great challenge for a long time. In the present study, we realize a directional proton migration through the construction of oriented bacteriorhodopsin (BR) microcapsules coated by FoF1-ATPase molecular motors through layer-by-layer (LBL) assembly. The changes in the conformation of BR under illumination lead to proton transfer in a radial direction, which generates a higher proton gradient to drive the synthesis of adenosine triphosphate (ATP) by FoF1-ATPase. Furthermore, to promote the photosynthetic activity, optically matched quantum dots were introduced into the artificial coassembly system of BR and FoF1-ATPase. Such a design creates a new path for the use of light energy.

引用

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