Evaluation of the reducing power effect on the enantioselective synthesis of methyl (R)-2-chloromandelate using Saccharomyces cerevisiae

被引：0

作者：

Jeong, Min ^{[1
]}

Yoo, Ik-keun ^{[1
]}

Han, Mee Jung ^{[2
]}

Hong, Soon Ho ^{[1
]}

机构：

[1] Univ Ulsan, Dept Chem Engn & Bioengn, Ulsan 680749, South Korea

[2] Dongyang Univ, Sch Chem & Biomol Engn, Pungki Eup 750711, Youngju, South Korea

来源：

KOREAN JOURNAL OF CHEMICAL ENGINEERING | 2011年 / 28卷 / 12期

关键词：

Asymmetric Reduction; Reducing Power; Methyl (R)-2-Chloromandelate; Saccharomyces cerevisiae; ASYMMETRIC REDUCTION; BICYCLIC DIKETONE; ESCHERICHIA-COLI; BIOCATALYSIS; DRUGS;

D O I：

10.1007/s11814-011-0124-3

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The effect of the reducing power on the reduction of methyl-2-chlorobenzoylformate was evaluated by using carbon substrates with different reducing powers. Glucose, sorbitol, and gluconate regenerated 2, 3, and 1 NAD(P)H during its conversion to pyruvate, respectively. When sorbitol was used as the carbon substrate, complete conversion was achieved in 8 h while it took 12 h and 19 h when glucose and gluconate were used, respectively. The enantiomeric excess (ee) value was 96.7% when sorbitol was used.

引用

页码：2316 / 2318

页数：3