Diagnosis of tuberculosis based on the detection of a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor by immuno-PCR

被引：13

作者：

Mehta, Promod K. ^{[1
]}

Singh, Netrapal ^{[1
]}

Dharra, Renu ^{[1
]}

Dahiya, Bhawna ^{[1
]}

Sharma, Suman ^{[1
]}

Sheoran, Abhishek ^{[2
]}

Gupta, Krishna B. ^{[3
]}

Chaudhary, Dhruva ^{[4
]}

Mehta, Neeru ^{[5
]}

Varma-Basil, Mandira ^{[6
]}

机构：

[1] Maharshi Dayanand Univ, Ctr Biotechnol, Rohtak 124001, Haryana, India

[2] MDU, Dept Stat, Rohtak, Haryana, India

[3] Univ Hlth Sci, Dept TB & Resp Med, Rohtak, Haryana, India

[4] UHS, Pulm & Crit Care Med, Rohtak, Haryana, India

[5] Ambedkar Inst Technol, Dept Med Elect, Delhi 110092, India

[6] Univ Delhi, VPCI, Delhi 110007, India

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2016年 / 127卷

关键词：

Tuberculosis; Immuno-PCR; Antigen; 85B; ESAT-6; Cord factor; BIOMARKERS;

D O I：

10.1016/j.mimet.2016.05.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Attempts were made to enhance the sensitivity of immuno-PCR assay based on the detection of cocktail of mycobacterial antigen 85B (Rv1886c), ESAT-6 (Rv3875) and cord factor (trehalose 6,6'-dimycolate) in pulmonary and extrapulmonary TB patients. Detection of Ag85B was found to be superior to the detection of cocktail in TB patients. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：24 / 27

页数：4

共 13 条

[1]

[Anonymous], 2009, WHO REPORT

[2] Systematic Review and Meta-Analysis of Antigen Detection Tests for the Diagnosis of Tuberculosis [J].

Flores, L. L. ;

Steingart, K. R. ;

Dendukuri, N. ;

Schiller, I. ;

Minion, J. ;

Pai, M. ;

Ramsay, A. ;

Henry, M. ;

Laal, S. .

CLINICAL AND VACCINE IMMUNOLOGY, 2011, 18 (10) :1616-1627

[3]

Hunt JD, 2001, ANTIGENIC COMPETITIO

[4] Serodiagnostic Efficacy of Mycobacterium tuberculosis 30/32-kDa Mycolyl Transferase Complex, ESAT-6, and CFP-10 in Patients with Active Tuberculosis [J].

Kumar, Gavish ;

Dagur, Pradeep Kumar ;

Singh, Prashant Kumar ;

Shankar, Hari ;

Yadav, Virendra S. ;

Katoch, Vishwa M. ;

Bajaj, Bharat ;

Gupta, Rajesh ;

Sengupta, Utpal ;

Joshi, Beenu .

ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS, 2010, 58 (01) :57-65

[5] Development of an ultrasensitive polymerase chain reaction-amplified immunoassay based on mycobacterial RD antigens: implications for the serodiagnosis of tuberculosis [J].

Mehta, Promod K. ;

Kalra, Mamta ;

Khuller, Gopal Krishan ;

Behera, Digambar ;

Verma, Indu .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 72 (02) :166-174

[6] Diagnostic Accuracy of a Rapid Urine Lipoarabinomannan Test for Tuberculosis in HIV-Infected Adults [J].

Nakiyingi, Lydia ;

Moodley, Vineshree Mischka ;

Manabe, Yukari C. ;

Nicol, Mark P. ;

Holshouser, Molly ;

Armstrong, Derek T. ;

Zemanay, Widaad ;

Sikhondze, Welile ;

Mbabazi, Olive ;

Nonyane, Bareng A. S. ;

Shah, Maunank ;

Joloba, Moses L. ;

Alland, David ;

Ellner, Jerrold J. ;

Dorman, Susan E. .

JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2014, 66 (03) :270-279

[7]

Rose C., 2012, J INFECT DIS IMMUN, V4, P36

[8] Evaluation of host Hsp(s) as potential biomarkers for the diagnosis of tuberculous meningitis [J].

Shekhawat, Seema D. ;

Purohit, Hemant J. ;

Taori, Girdhar M. ;

Daginawala, Hatim F. ;

Kashyap, Rajpal S. .

CLINICAL NEUROLOGY AND NEUROSURGERY, 2016, 140 :47-51

[9] Serodiagnostic potential of immuno-PCR using a cocktail of mycobacterial antigen 85B, ESAT-6 and cord factor in tuberculosis patients [J].

Singh, Netrapal ;

Sreenivas, Vishnubhatla ;

Sheoran, Abhishek ;

Sharma, Suman ;

Gupta, Krishna B. ;

Khuller, Gopal K. ;

Mehta, Promod K. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2016, 120 :56-64

[10] Diagnosis of pulmonary and extrapulmonary tuberculosis based on detection of mycobacterial antigen 85B by immuno-PCR [J].

Singh, Netrapal ;

Sreenivas, Vishnubhatla ;

Gupta, Krishna B. ;

Chaudhary, Anil ;

Mittal, Anshu ;

Varma-Basil, Mandira ;

Prasad, Rajendra ;

Gakhar, Surender K. ;

Khuller, Gopal K. ;

Mehta, Promod K. .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2015, 83 (04) :359-364

← 1 2 →