In Vitro Antibacterial Mechanism of High-Voltage Electrostatic Field against Acinetobacter johnsonii

被引：11
|
作者：
Huang, Han [1 ,2 ,3 ]
Gao, Tianqi [1 ,4 ]
Qian, Xiaoqing [1 ,4 ]
Wu, Wenjing [1 ]
Fan, Xiuzhi [1 ]
Shi, Liu [1 ]
Xiong, Guangquan [1 ]
Ding, Anzi [1 ]
Li, Xin [1 ]
Qiao, Yu [1 ]
Liao, Li [1 ]
Wang, Lan [1 ]
机构：
[1] Minist Agr & Rural Affairs, Inst Agroprod Proc & Nucl Agr Technol, Hubei Acad Agr Sci, Key Lab Agr Prod Cold Chain Logist, Wuhan 430064, Peoples R China
[2] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[3] Liangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[4] Hubei Univ Technol, Bioengn & Food Coll, Dept Biomed & Biopharmacol, Wuhan 430068, Peoples R China
来源：
FOODS | 2022年 / 11卷 / 07期
关键词：
Acinetobacter johnsonii; high-voltage static electric field; biochemical properties; stress-related genes; LISTERIA-MONOCYTOGENES; ESCHERICHIA-COLI; ENERGY-CONSUMPTION; ELECTRIC-FIELD; INACTIVATION; PLASMA; BACTERIAL; STRESS; CELLS;
D O I：
10.3390/foods11070955
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
This study aimed to investigate the antibacterial properties and mechanisms of a high-voltage static electric field (HVEF) in Acinetobacter johnsonii, which were assessed from the perspective of biochemical properties and stress-related genes. The time/voltage-kill assays and growth curves showed that an HVEF decreased the number of bacteria and OD600 values. In addition, HVEF treatment caused the leakage of cell contents (nucleic acids and proteins), increased the electrical conductivity and amounts of reactive oxygen substances (ROS) (16.88 fold), and decreased the activity of Na+ K+-ATPase in A. johnsonii. Moreover, the changes in the expression levels of genes involved in oxidative stress and DNA damage in the treated A. johnsonii cells suggested that HVEF treatment could induce oxidative stress and DNA sub-damage. This study will provide useful information for the development and application of an HVEF in food safety.
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页数：12
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