IGFBP-3 and IGFBP-5 production by human intestinal muscle:: reciprocal regulation by endogenous TGF-β1

被引：32
作者：
Bushman, TL [1 ]
Kuemmerle, JF [1 ]
机构：
[1] Virginia Commonwealth Univ, Dept Med, Div Gastroenterol, Richmond, VA 23298 USA
来源：
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1998年 / 275卷 / 06期
关键词：
smooth muscle cells; insulin-like growth factor-1; proliferation; insulin-like growth factor binding proteins; transforming growth factor-beta 1;
D O I：
10.1152/ajpgi.1998.275.6.G1282
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Insulin-like growth factor-I (IGF-I)-mediated growth of cells can be modulated by specific IGF binding proteins (IGFBPs) that inhibit or augment IGF-I ligand-receptor interaction. IGFBP expression and production by human intestinal muscle cells in culture was characterized in rapidly growing cells (day 3 of culture), in confluent cells (day 7), and in postconfluent cells (day 14). RT-PCR analysis identified IGFBP-3, IGFBP-4, and IGFBP-5 mRNA during all three phases of growth. The production of IGFBP-3 and IGFBP-5 was regulated in reciprocal fashion. IGFBP-5 production was high on day 3 and decreased two- to fivefold by day 14, and IGFBP-3 production was low on day 3 and increased five- to eightfold by day 14. IGFBP-4 production remained constant. IGFBP-3 inhibited and IGFBP-5 augmented IGF-I-induced proliferation. IGFBP-3 and IGFBP-5 production was regulated in reciprocal fashion by transforming growth factor-beta 1 (TGF-beta 1) Immunoneutralization of endogenous TGF-beta 1 decreased the production of IGFBP-3 and increased the production of IGFBP-5. Addition of exogenous recombinant human TGF-beta 1 had the opposite effect. We conclude that the expression and time-dependent production of IGFBP-3, IGFBP-4, and IGFBP-5 and their regulation by endogenous TGF-beta 1 represent mechanisms by which human intestinal muscle cells regulate autocrine IGF-I-mediated growth.
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收藏
页码：G1282 / G1290
页数：9
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