Porcine Reproductive and Respiratory Syndrome (PRRS) Epidemiology in an Integrated Pig Company of Northern Italy: A Multilevel Threat Requiring Multilevel Interventions

被引:13
作者
Franzo, Giovanni [1 ]
Barbierato, Giacomo [1 ]
Pesente, Patrizia [2 ]
Legnardi, Matteo [1 ]
Tucciarone, Claudia Maria [1 ]
Sandri, Giampietro [2 ]
Drigo, Michele [1 ]
机构
[1] Univ Padua, Dept Anim Med Prod & Hlth MAPS, I-35020 Padua, Italy
[2] Lab Tre Valli, I-37132 Verona, Italy
来源
VIRUSES-BASEL | 2021年 / 13卷 / 12期
关键词
porcine reproductive and respiratory syndrome; molecular epidemiology; ORF7; phylodynamics; phylogeography; Italy; evolution; pig flows; SYNDROME VIRUS PRRSV; DISTANCE AIRBORNE TRANSPORT; SEQUENCES; ALGORITHM; ALIGNMENT; MOVEMENT; GENOTYPE; SPREAD; REGION; MODEL;
D O I
10.3390/v13122510
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Porcine reproductive and respiratory syndrome (PRRS) is probably the most relevant viral disease affecting pig farming. Despite the remarkable efforts paid in terms of vaccination administration and biosecurity, eradication and long-term control have often been frustrated. Unfortunately, few studies are currently available that objectively link, using a formal statistical approach, viral molecular epidemiology to the risk factors determining the observed scenario. The purpose of the present study is to contribute to filling this knowledge gap taking advantage of the advancements in the field of phylodynamics. Approximately one-thousand ORF7 sequences were obtained from strains collected between 2004 and 2021 from the largest Italian pig company, which implements strict compartmentalization among independent three-sites (i.e., sow herds, nurseries and finishing units) pig flows. The history and dynamics of the viral population and its evolution over time were reconstructed and linked to managerial choices. The viral fluxes within and among independent pig flows were evaluated, and the contribution of other integrated pig companies and rurally risen pigs in mediating such spreading was investigated. Moreover, viral circulation in Northern Italy was reconstructed using a continuous phylogeographic approach, and the impact of several environmental features on PRRSV strain persistence and spreading velocity was assessed. The results demonstrate that PRRSV epidemiology is shaped by a multitude of factors, including pig herd management (e.g., immunization strategy), implementation of strict-independent pig flows, and environmental features (e.g., climate, altitude, pig density, road density, etc.) among the others. Small farms and rurally raised animals also emerged as a potential threat for larger, integrated companies. These pieces of evidence suggest that none of the implemented measures can be considered effective alone, and a multidimensional approach, ranging from individual herd management to collaboration and information sharing among different companies, is mandatory for effective infection control.
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页数:17
相关论文
共 54 条
[1]   TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations [J].
Abascal, Federico ;
Zardoya, Rafael ;
Telford, Maximilian J. .
NUCLEIC ACIDS RESEARCH, 2010, 38 :W7-W13
[2]   The role of animal movement, including off-farm rearing of heifers, in the interherd transmission of multidrug-resistant Salmonella [J].
Adhikari, B. ;
Besser, T. E. ;
Gay, J. M. ;
Fox, L. K. ;
Davis, M. A. ;
Cobbold, R. N. ;
Berge, A. C. B. ;
Hancock, D. D. .
JOURNAL OF DAIRY SCIENCE, 2009, 92 (09) :4229-4238
[3]   Novel approaches for Spatial and Molecular Surveillance of Porcine Reproductive and Respiratory Syndrome Virus (PRRSv) in the United States [J].
Alkhamis, Moh A. ;
Arruda, Andreia G. ;
Morrison, Robert B. ;
Perez, Andres M. .
SCIENTIFIC REPORTS, 2017, 7
[4]   Land altitude, slope, and coverage as risk factors for Porcine Reproductive and Respiratory Syndrome (PRRS) outbreaks in the United States [J].
Arruda, Andreia Goncalves ;
Vilalta, Carles ;
Perez, Andres ;
Morrison, Robert .
PLOS ONE, 2017, 12 (04)
[5]   Improving the Accuracy of Demographic and Molecular Clock Model Comparison While Accommodating Phylogenetic Uncertainty [J].
Baele, Guy ;
Lemey, Philippe ;
Bedford, Trevor ;
Rambaut, Andrew ;
Suchard, Marc A. ;
Alekseyenko, Alexander V. .
MOLECULAR BIOLOGY AND EVOLUTION, 2012, 29 (09) :2157-2167
[6]   BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis [J].
Bouckaert, Remco ;
Vaughan, Timothy G. ;
Barido-Sottani, Joelle ;
Duchene, Sebastian ;
Fourment, Mathieu ;
Gavryushkina, Alexandra ;
Heled, Joseph ;
Jones, Graham ;
Kuehnert, Denise ;
De Maio, Nicola ;
Matschiner, Michael ;
Mendes, Fabio K. ;
Mueller, Nicola F. ;
Ogilvie, Huw A. ;
du Plessis, Louis ;
Popinga, Alex ;
Rambaut, Andrew ;
Rasmussen, David ;
Siveroni, Igor ;
Suchard, Marc A. ;
Wu, Chieh-Hsi ;
Xie, Dong ;
Zhang, Chi ;
Stadler, Tanja ;
Drummond, Alexei J. .
PLOS COMPUTATIONAL BIOLOGY, 2019, 15 (04)
[7]  
Candotti P., 2006, 19 IPVS C COP DENM, V1, P54
[8]   Phenotypic characterization of a highly pathogenic Italian porcine reproductive and respiratory syndrome virus (PRRSV) type 1 subtype 1 isolate in experimentally infected pigs [J].
Canelli, Elena ;
Catella, Alessia ;
Borghetti, Paolo ;
Ferrari, Luca ;
Ogno, Giulia ;
De Angelis, Elena ;
Corradi, Attilio ;
Passeri, Benedetta ;
Bertani, Valeria ;
Sandri, Giampietro ;
Bonilauri, Paolo ;
Leung, Frederick C. ;
Guazzetti, Stefano ;
Martelli, Paolo .
VETERINARY MICROBIOLOGY, 2017, 210 :124-133
[9]   Commercial PRRS Modified-Live Virus Vaccines [J].
Chae, Chanhee .
VACCINES, 2021, 9 (02) :1-16
[10]   Porcine reproductive and respiratory syndrome virus [J].
Cho, Jenny G. ;
Dee, Scott A. .
THERIOGENOLOGY, 2006, 66 (03) :655-662