Demonstration of Hollow Fiber Membrane-Based Enclosed Space Air Remediation for Capture of an Aerosolized Synthetic SARS-CoV-2 Mimic and Pseudovirus Particles

Reduction of airborne viral particles in enclosed spaces is critical in controlling pandemics. Three different hollow fiber membrane (HFM) modules were investigated for viral aerosol separation in enclosed spaces. Pore structures were characterized by scanning electron microscopy, and air transport properties were measured. Particle removal efficiency was characterized using aerosols generated by a collision atomizer from a defined mixture of synthetic nanoparticles including SARS-CoV-2 mimics (protein-coated 100 nm polystyrene). HFM1 (polyvinylidene fluoride, similar to 50-1300 nm pores) demonstrated 96.5-100% efficiency for aerosols in the size range of 0.3-3 at a flow rate of 18.6 +/- 0.3 SLPM (1650 LMH), whereas HFM2 (polypropylene, 9-40 nm pores) and HFM3 (hydrophilized polyether sulfone, similar to 140-750 nm pores) demonstrated 99.65-100% and 98.8-100% efficiency at flow rates of 19.7 +/- 0.3 SLPM (similar to 820 LMH) and 19.4 +/- 0.2 SLPM (similar to 4455 LMH), respectively. Additionally, lasting filtration with minimal fouling was demonstrated using ambient aerosols over 2 days. Finally, each module was evaluated with pseudovirus (vesicular stomatitis virus) aerosol, demonstrating 99.3% (HFM1), >99.8% (HFM2), and >99.8% (HFM3) reduction in active pseudovirus titer as a direct measure of viral particle removal. These results quantified the aerosol separation efficiency of HFMs and highlight the need for further development of this technology to aid the fight against airborne viruses and particulate matter concerning human health.