In Vivo Dual-Modality Fluorescence and Magnetic Resonance Imaging-Guided Lymph Node Mapping with Good Biocompatibility Manganese Oxide Nanoparticles

被引：22

作者：

Zhan, Yonghua ^{[1
,2
]}

Zhan, Wenhua ^{[3
,4
]}

Li, Hanrui ^{[1
]}

Xu, Xinyi ^{[1
]}

Cao, Xu ^{[1
]}

Zhu, Shouping ^{[1
]}

Liang, Jimin ^{[1
]}

Chen, Xueli ^{[1
]}

机构：

[1] Xidian Univ, Sch Life Sci & Technol, Engn Res Ctr Mol & Neuro Imaging, Minist Educ, Xian 710071, Shaanxi, Peoples R China

[2] Xidian Univ, Xidian Ningbo Informat Technol Inst, Xian 710071, Shaanxi, Peoples R China

[3] Ningxia Med Univ, Dept Radiotherapy, Gen Hosp, Ningxia 750004, Peoples R China

[4] Xi An Jiao Tong Univ, Sch Life Sci & Technol, Key Lab Biomed Informat Engn, Educ Minist, Xian 710049, Shaanxi, Peoples R China

来源：

MOLECULES | 2017年 / 22卷 / 12期

基金：

中国国家自然科学基金;

关键词：

manganese oxide nanoparticles; fluorescence imaging; magnetic resonance imaging; lymph node; dual-modality; QUANTUM DOTS; MOLECULAR-MECHANISMS; MNO NANOPARTICLES; CANCER METASTASIS; TUMOR-METASTASIS; CONTRAST AGENTS; MRI CONTRAST; LYMPHANGIOGENESIS; NANOPROBES; NANOCRYSTALS;

D O I：

10.3390/molecules22122208

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multifunctional manganese oxide nanoparticles (NPs) with impressive enhanced T-1 contrast ability show great promise in biomedical diagnosis. Herein, we developed a dual-modality imaging agent system based on polyethylene glycol (PEG)-coated manganese oxide NPs conjugated with organic dye (Cy-7.5), which functions as a fluorescence imaging (FI) agent as well as a magnetic resonance imaging (MRI) imaging agent. The formed Mn3O4@PEG-Cy-7.5 NPs with the size of similar to 10 nm exhibit good colloidal stability in different physiological media. Serial FI and MRI studies that non-invasively assessed the bio-distribution pattern and the feasibility for in vivo dual-modality imaging-guided lymph node mapping have been investigated. In addition, histological and biochemical analyses exhibited low toxicity even at a dose of 20 mg/kg in vivo. Since Mn3O4@PEG-Cy-7.5 NPs exhibited desirable properties as imaging agents and good biocompatibility, this work offers a robust, safe, and accurate diagnostic platform based on manganese oxide NPs for tumor metastasis diagnosis.

引用

页数：13

共 36 条

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