Are brucellosis, Q fever and melioidosis potential causes of febrile illness in Madagascar?

被引：10

作者：

Boone, Ides ^{[1
]}

Henning, Klaus ^{[2
]}

Hilbert, Angela ^{[2
,10
]}

Neubauer, Heinrich ^{[2
]}

Von Kalckreuth, Vera ^{[3
]}

Dekker, Denise Myriam ^{[4
]}

Schwarz, Norbert Georg ^{[4
]}

Pak, Gi Deok ^{[3
]}

Kruger, Andreas ^{[5
]}

Hagen, Ralf Matthias ^{[5
]}

Frickmann, Hagen ^{[5
,6
]}

Heriniaina, Jean Noel ^{[7
]}

Rakotozandrindrainy, Raphael ^{[7
]}

Rakotondrainiarivelo, Jean Philibert ^{[7
]}

Razafindrabe, Tsiry ^{[7
]}

Hogan, Benedikt ^{[4
]}

May, Juergen ^{[4
]}

Marks, Florian ^{[3
]}

Poppert, Sven ^{[4
,8
]}

Al Dahouk, Sascha ^{[1
,9
]}

机构：

[1] German Fed Inst Risk Assessment, Dept Biol Safety, Diedersdorfer Weg 1, D-12277 Berlin, Germany

[2] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Bacterial Infect & Zoonoses, Naumburger Str 96a, D-07743 Jena, Germany

[3] Int Vaccine Inst, SNU Res Pk,1 Gwanak Ro, Seoul 08226, South Korea

[4] Bernhard Nocht Inst Trop Med, Bernhard Nocht Str 74, D-20359 Hamburg, Germany

[5] Bundeswehr Hosp Hamburg, Bernhard Nocht Inst, Dept Trop Med, Bernhard Nocht Str 74, D-20359 Hamburg, Germany

[6] Univ Med Rostock, Schillingallee 70, D-18057 Rostock, Germany

[7] Univ Antananarivo, Dept Microbiol & Parasitol, BP 175, Antananarivo, Madagascar

[8] Univ Med Ctr Hamburg Eppendorf, Martinistr 52, D-20246 Hamburg, Germany

[9] RWTH Aachen Univ Hosp, Pauwelsstr 30, D-52074 Aachen, Germany

[10] Landkreis Havelland, Amt Landwirtschaft Vet & Lebensmitteluberwachung, Goethestr 59-60, D-14641 Nauen, Germany

来源：

ACTA TROPICA | 2017年 / 172卷

关键词：

Zoonoses; Brucellosis; Q fever; Melioidosis; Febrile illness; Madagascar; REAL-TIME PCR; COXIELLA-BURNETII; BURKHOLDERIA-PSEUDOMALLEI; DAIRY-CATTLE; IDENTIFICATION; PREVALENCE; EPIDEMIOLOGY; AFRICA; ASSAYS; ANTIBODIES;

D O I：

10.1016/j.actatropica.2017.05.013

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Brucellosis, Q fever and melioidosis are zoonoses, which can lead to pyrexia. These diseases are often under ascertained and underreported because of their unspecific clinical signs and symptoms, insufficient awareness by physicians and public health officers and limited diagnostic capabilities, especially in low-resource countries. Therefore, the presence of Brucella spp., Coxiella burnetii and Burkholderia pseudomallei was investigated in Malagasy patients exhibiting febrile illness. In addition, we analyzed zebu cattle and their ticks as potential reservoirs for Brucella and C. burnetii, respectively. Specific quantitative real-time PCR assays (qPCRs) were performed on 1020 blood samples drawn from febrile patients. In total, 15 samples (1.5%) were Brucella-positive, mainly originating from patients without travel history, while DNA from C. burnetii and Bu. pseudomallei was not detected. Anti-C. burnetii antibodies were found in four out of 201 zebu serum samples (2%), whereas anti-Brucella antibodies could not be detected. Brucella DNA was detected in a single zebu sample. Three out of 330 ticks analyzed (1%) were positively tested for C. burnetii DNA but with high Ct values in the qPCR assay. Our data suggest that zebus as well as Amblyomma and Boophilus ticks have to be considered as a natural reservoir or vector for C. burnetii, but the risk of cattle -to -human transmission is low. Since bovine brucellosis does not seem to contribute to human infections in Madagascar, other transmission routes have to be assumed.

引用

页码：255 / 262

页数：8

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