Enzymatic hydrolysis of hemicelluloses from Miscanthus to monosaccharides or xylo-oligosaccharides by recombinant hemicellulases

被引：52

作者：

Li, Hailong ^{[1
,2
]}

Xue, Yong ^{[1
]}

Wu, Jinlian ^{[1
]}

Wu, Hongli ^{[1
]}

Qin, Guanghui ^{[1
]}

Li, Chengguo ^{[1
]}

Ding, Jiajia ^{[1
]}

Liu, Jian ^{[1
]}

Gan, Lihui ^{[1
]}

Long, Minnan ^{[1
]}

机构：

[1] Xiamen Univ, Coll Energy, Xiamen 361102, Peoples R China

[2] Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2016年 / 79卷

基金：

中国国家自然科学基金;

关键词：

Hemicelluloses; Miscanthus; Monosaccharides; Recombinant hemicellulases; Xylo-oligosaccharides; GRADED ETHANOL PRECIPITATION; SWITCHGRASS PANICUM-VIRGATUM; L; XYLAN; DEGRADATION; DELIGNIFICATION; ENZYMES; BIOMASS; DIGESTIBILITY; BIOCONVERSION; PRETREATMENT;

D O I：

10.1016/j.indcrop.2015.11.021

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Hemicelluloses isolated from holocellulose (MHOC) from Miscanthus were characterized by RP-HPLC-UV, FT-IR, and NMR. The hemicelluloses and recombinant hemicellulases, including endo-beta-1,4-xylanases (HoXyn11A and AnXyn10C), beta-xylosidases (AnXln3D), and alpha-L-arabinofuranosidases (AnAxh62A), as well as their interaction mechanisms were investigated by enzymatic hydrolysis. AnXyn10C released shorter end products than HoXyn11A from isolated hemicelluloses (IHEC). AnAxh62A was able to release all single-substituted alpha-L-arabinofuranosyl residues from IHEC. AnXynl10C and HoXyn11A were able to directly act on MHOC, whereas AnAxh62A and AnXln3D did not. The combination of HoXyn11A and AnAxh62A produced the highest xylo-oligosaccharides (XOS) yield from IHEC, whereas AnXynl10C alone produced the highest XOS yield from MHOC. The combination of HoXyn11A, AnAxh62A, and AnXln3D achieved the highest xylose yield from IHEC, whereas the combination of AnXyn10C, AnAxh62A, and AnXln3D achieved the highest xylose yield from MHOC. This study contributes to the development of efficient enzyme cocktails for the bioconversion of hemicelluloses from Miscanthus into monosaccharides and XOS. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：170 / 179

页数：10

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