Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway

被引：124

作者：

Beaudoin, F ^{[1
]}

Michaelson, LV ^{[1
]}

Hey, SJ ^{[1
]}

Lewis, MJ ^{[1
]}

Shewry, PR ^{[1
]}

Sayanova, O ^{[1
]}

Napier, JA ^{[1
]}

机构：

[1] Univ Bristol, Long Ashton Res Stn, Dept Agr Sci, Inst Arable Crops Res, Bristol BS41 9AF, Avon, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 12期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1073/pnas.110140197

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A Caenorhabditis elegans ORF encoding the presumptive condensing enzyme activity of a fatty acid elongase has been characterized functionally by heterologous expression in yeast. This ORF (F56H 11.4) shows low similarity to Saccharomyces cerevisiae genes involved in fatty acid elongation. The substrate specificity of the C. elegans enzyme indicated a preference for Delta(6)-desaturated C18 polyunsaturated fatty acids. Coexpression of this activity with fatty acid desaturases required for the synthesis of C20 polyunsaturated fatty acids resulted in the accumulation of arachidonic acid from linoleic acid and eicosapentaenoic acid from alpha-linolenic acid. These results demonstrate the reconstitution of the n-3 and n-6 polyunsaturated fatty acid biosynthetic pathways. The C. elegans ORF is likely to interact with endogenous components of a yeast elongation system, with the heterologous nematode condensing enzyme F56H11.4 causing a redirection of enzymatic activity toward polyunsaturated C18 fatty acid substrates.

引用

页码：6421 / 6426

页数：6

共 27 条

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