Anticipating and Identifying Collateral Damage in Genome Editing

被引：27
作者：
Burgio, Gaetan [1 ]
Teboul, Lydia [2 ]
机构：
[1] Australian Natl Univ, John Curtin Sch Med Res, Dept Immunol & Infect Dis, Canberra, ACT 2603, Australia
[2] MRC, Mary Lyon Ctr, Harwell Inst, Harwell Campus, Didcot OX11 0RD, Oxon, England
来源：
TRENDS IN GENETICS | 2020年 / 36卷 / 12期
基金：
中国国家自然科学基金; 英国医学研究理事会; 澳大利亚研究理事会;
关键词：
TARGET CLEAVAGE; CRISPR CAS9; DNA; GENE; MICE; GENERATION; MUTATIONS; SEQ; EFFICIENT; IMMUNITY;
D O I：
10.1016/j.tig.2020.09.011
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Genome editing has powerful applications in research, healthcare, and agriculture. However, the range of possible molecular events resulting from genome editing has been underestimated and the technology remains unpredictable on, and away from, the target locus. This has considerable impact in providing a safe approach for therapeutic genome editing, agriculture, and other applications. This opinion article discusses how to anticipate and detect those editing events by a combination of assays to capture all possible genomic changes. It also discusses strategies for preventing unwanted effects, critical to appraise the benefit or risk associated with the use of the technology. Anticipating and verifying the result of genome editing are essential for the success for all applications.
引用
收藏
页码：905 / 914
页数：10
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