Whole Genome Analysis of Sugarcane Root-Associated Endophyte Pseudomonas aeruginosa B18-A Plant Growth-Promoting Bacterium With Antagonistic Potential Against Sporisorium scitamineum

被引：67

作者：

Singh, Pratiksha ^{[1
,2
]}

Singh, Rajesh Kumar ^{[1
,2
]}

Guo, Dao-Jun ^{[1
,2
,3
]}

Sharma, Anjney ^{[1
,2
]}

Singh, Ram Nageena ^{[4
]}

Li, Dong-Ping ^{[5
]}

Malviya, Mukesh K. ^{[1
,2
]}

Song, Xiu-Peng ^{[2
]}

Lakshmanan, Prakash ^{[1
,6
,7
]}

Yang, Li-Tao ^{[2
,3
]}

Li, Yang-Rui ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Agr Sci, Guangxi Acad Agr Sci, Minist Agr,Sugarcane Res Ctr,Key Lab Sugarcane Bi, Guangxi Key Lab Sugarcane Genet Improvement Sugar, Nanning, Peoples R China

[2] Guangxi Key Lab Crop Genet Improvement & Biotechn, Nanning, Peoples R China

[3] Guangxi Univ, Coll Agr, Nanning, Peoples R China

[4] AgriGenome Labs Pvt Ltd, Kochi, Kerala, India

[5] Guangxi Acad Agr Sci, Microbiol Inst, Nanning, Peoples R China

[6] Southwest Univ, Coll Resources & Environm, Interdisciplinary Res Ctr Agr Green Dev Yangtze R, Chongqing, Peoples R China

[7] Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld, Australia

来源：

FRONTIERS IN MICROBIOLOGY | 2021年 / 12卷

基金：

中国国家自然科学基金;

关键词：

biocontrol; biotic stress; endophyte; genome sequence; plant growth promotion; Pseudomonas aeruginosa; sugarcane; INDUCED SYSTEMIC RESISTANCE; ARACHIS-HYPOGAEA; GENE-EXPRESSION; DIVERSITY; ASSAY; FIELD; RHIZOBACTERIA; SIDEROPHORES; LIPOPEPTIDES; DEAMINASE;

D O I：

10.3389/fmicb.2021.628376

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sugarcane smut is a significant fungal disease that causes a major loss in sugar yield and quality. In this study, we isolated an endophytic strain B18 from a sugarcane root, which showed plant growth-promotion, hydrolytic enzyme production, antifungal activity against sugarcane pathogens (Sporisorium scitamineum, Ceratocystis paradoxa, Fusarium verticillioides), and the presence of nifH, acdS, and antibiotic genes (hcn, prn, and phCA) under in vitro conditions. BIOLOG((R)) phenotypic profiling of B18 established its ability to use various carbon and nitrogen sources and tolerate a range of pH and osmotic and temperature stresses. Whole-genome analysis of B18, identified as Pseudomonas aeruginosa, showed that it consists of a single circular chromosome of 6,490,014 bp with 66.33% GC content. Genome annotation has identified 5,919 protein-coding genes, and 65 tRNA, and 12 rRNA genes. The P. aeruginosa B18 genome encodes genes related to ethylene, nitrogen (nifU, norBCDERQ, gltBDPS, and aatJMPQ), and phosphate (pstABCS and phoBDHRU) metabolism and produce indole-3-acetic acid and siderophores. This also includes genes encoding hydrolases and oxidoreductases, those associated with biocontrol mechanisms (hcnABC, phzA_B, phzDEFGMS, and pchA), colonization (minCDE and lysC), and biofilm formation (efp, hfq, flgBCDEFGHI, and motAB), and those associated with metabolism of secondary metabolites. Collectively, these results suggest a role for P. aeruginosa B18 in plant growth enhancement and biocontrol mechanisms. The P. aeruginosa B18 strain was found to be an efficient colonizer in sugarcane; it can improve growth through modulation of plant hormone production and enhanced host-plant resistance to smut pathogen S. scitamineum in a smut-susceptible sugarcane variety (Yacheng71-374). These biocontrol and plant growth promotion properties of P. aeruginosa B18 area are discussed in this report.

引用

页数：21

共 118 条

[1] Siderophores in environmental research: roles and applications [J].