L-Dopa Synthesis on Conducting Polymers

被引：8

作者：

Erdogan, Huriye ^{[1
]}

Tuncagil, Sevinc ^{[1
]}

Toppare, Levent ^{[1
]}

机构：

[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

来源：

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY | 2010年 / 47卷 / 03期

关键词：

Conducting polymers; electrochemical biosensors; enzyme immobilization; L-dopa synthesis; tyrosinase; IMMOBILIZED TYROSINASE; POLYPHENOL OXIDASE; PHENOLIC-COMPOUNDS; GLUCOSE-OXIDASE; COPOLYMERS; PERFORMANCE; INVERTASE; ACID;

D O I：

10.1080/10601320903526865

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

With regards to the synthesis of L-Dopa (l-3,4-dihydroxy phenylalanine) two types of biosensors were designed by immobilizing tyrosinase on conducting polymers; polypyrrole (PPy) and poly(3,4-ethylenedioxythiophene) (PEDOT). PPy and PEDOT were synthesized electrochemically and tyrosinase immobilized by entrapment during electropolymerization. The kinetic parameters of the designed biosensors, maximum reaction rate of the enzyme (Vmax) and Michaelis Menten constant (Km) were determined. Vmax were found as 0.013 for PPy matrix and 0.041 mol/min.electrode for PEDOT matrix. Km values were determined as 3.7 and 5.2mM for PPy and PEDOT matrices respectively. Calibration curves for enzyme activity vs. substrate concentration were drawn for the range of 0.8 to 2.5 mM L-Tyrosine. Optimum temperature and pH, operational and shelf life stabilities of immobilized enzyme were also examined.

引用

页码：209 / 214

页数：6

共 40 条

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