Surface Acoustic Waves Sensor for DNA-Biosensor Development

被引：17

作者：

Fourati, Najla ^{[2
]}

Lazerges, Mathieu ^{[1
]}

Vedrine, Christophe ^{[1
]}

Fougnion, Jean-Marie ^{[2
]}

Zerrouki, Chouki ^{[2
]}

Rousseau, Lionel ^{[3
]}

Lepeut, Patrick ^{[2
]}

Bonnet, Jean Jacques ^{[2
]}

Pernelle, Christine ^{[1
]}

机构：

[1] Conservatoire Natl Arts & Metiers, Lab Genie Analyt, EA 4131, F-75003 Paris, France

[2] Conservatoire Natl Arts & Metiers, Phys Lab, EA 4131, F-75003 Paris, France

[3] ESIEE, ELMI EA 4131, Serv Moyens Microelect, F-93162 Noisy Le Grand, France

来源：

SENSOR LETTERS | 2009年 / 7卷 / 05期

关键词：

Surface Acoustic Waves Sensor; LiTaO3; Piezoelectric; Biosensor; DNA; QUARTZ-CRYSTAL MICROBALANCE; CHEMICAL-ANALYSIS; GMOS DETECTION; HYBRIDIZATION; IMMOBILIZATION;

D O I：

10.1166/sl.2009.1160

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A 104 MHz LiTaO3 shear horizontal surface acoustic waves sensor (SH-SAW), working in a dual delay line configuration, was used to design a DNA-biosensor. Grafting of 27-mer oligonucleotide probes on the gold covered LiTaO3 crystal surface, and successive hybridization cycles with 36-mer oligonucleotide targets, were successfully achieved. Grafting and hybridization efficiency measured are consistent with those reported with other physical sensors. The device mass sensitivity, of 6.3 Hz/ng, is in the range of quartz microbalance bulk acoustic wave sensor (QCM-BAW) detection limit. Technical development of SH-SAW sensors is a major challenge for label-free DNA detection, as such devices, widely used in mobile and wireless communications, can reach several GHz frequencies.

引用

页码：847 / 850

页数：4

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