Analysis of Inflammatory Cytokines in the Tears of Dry Eye Patients

被引:370
作者
Massingale, Morgan L. [1 ]
Li, Xiaohong [1 ]
Vallabhajosyula, Maithreyi [1 ]
Chen, Dongmei [1 ]
Wei, Yi [1 ]
Asbell, Penny A. [1 ]
机构
[1] Mt Sinai Sch Med, Dept Ophthalmol, New York, NY 10029 USA
关键词
cytokines; inflammation; dry eye disease; Multiplex bead immunoassays; real time RT-PCR; CYCLOSPORINE OPHTHALMIC EMULSION; OCULAR SURFACE; KERATOCONJUNCTIVITIS SICCA; CONJUNCTIVAL EPITHELIUM; INTERLEUKIN-6; LEVELS; SJOGRENS-SYNDROME; GENE-EXPRESSION; BEHCETS-DISEASE; WORKSHOP; 2007; SUBCOMMITTEE;
D O I
10.1097/ICO.0b013e3181a16578
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: To determine the levels of 8 important cytokines and 1 chemokine in tears of patients with dry eye disease. Methods: Tear samples were collected from 7 patients with dry eye disease and 7 healthy volunteers, and impression cytology samples were collected from 3 of the dry eye patients and 3 of the normal controls. Tears were analyzed for the presence of 8 cytokines [interleukin (IL)-2, IL-4, IL-5, IL-6, IL-10, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, IL-1 beta] and 1 chemokine (IL-8). The cytokines and chemokine in each tear sample were measured using Invitrogen's Multiplex Bead Immunoassays. The impression cytology samples were analyzed for IL-1 beta, IL-6, IL-8, and TNF-alpha mRNA expression using real-time reverse transcriptase polymerase chain reaction anlaysis. Results: All cytokines and the chemokine measured were significantly increased in the tears of dry eye patients as compared to normal controls. mRNA of all four markers was increased, and the fold increase correlated well with the fold increase of the cytokine concentration found in the tear samples. Conclusion: Tears from dry eye patients contain significantly increased concentrations of cytokines that show correlation to severity of the disease. The upregulation of their respective genes in the conjunctiva suggests that the concentration increase is not the result of evaporative effects, but of overproduction. These findings suggest that cytokines may play an important role in dry eye disease and topical cytokine modulators may be explored as a therapeutic approach to dry eye disease.
引用
收藏
页码:1023 / 1027
页数:5
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