Nonalcoholic fatty liver disease is associated with dysbiosis independent of body mass index and insulin resistance

被引：222

作者：

Da Silva, Hannah E. ^{[1
]}

Teterina, Anastasia ^{[2
]}

Comelli, Elena M. ^{[3
]}

Taibi, Amel ^{[3
]}

Arendt, Bianca M. ^{[2
]}

Fischer, Sandra E. ^{[2
]}

Lou, Wendy ^{[4
]}

Allard, Johane P. ^{[2
,3
]}

机构：

[1] Sunnybrook Hlth Sci Ctr, Toronto, ON, Canada

[2] Univ Toronto, Univ Hlth Network, Toronto Gen Hosp, Dept Med, Toronto, ON, Canada

[3] Univ Toronto, Dept Nutr Sci, Toronto, ON, Canada

[4] Univ Toronto, Dalla Lana Sch Publ Hlth, Toronto, ON, Canada

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

加拿大健康研究院;

关键词：

GUT MICROBIOTA; INTESTINAL MICROBIOTA; METABOLIC SYNDROME; STEATOHEPATITIS; INFLAMMATION; ABSORPTION; COMMUNITY; SEVERITY; GLUCOSE; HUMANS;

D O I：

10.1038/s41598-018-19753-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study aimed to determine if there is an association between dysbiosis and nonalcoholic fatty liver disease (NAFLD) independent of obesity and insulin resistance (IR). This is a prospective cross-sectional study assessing the intestinal microbiome (IM) of 39 adults with biopsy-proven NAFLD (15 simple steatosis [SS]; 24 nonalcoholic steatohepatitis [NASH]) and 28 healthy controls (HC). IM composition (llumina MiSeq Platform) in NAFLD patients compared to HC were identified by two statistical methods (Metastats, Wilcoxon). Selected taxa was validated using quantitative PCR (qPCR). Metabolites in feces and serum were also analyzed. In NAFLD, 8 operational taxonomic units, 6 genera, 6 families and 2 phyla (Bacteroidetes, Firmicutes) were less abundant and; 1 genus (Lactobacillus) and 1 family (Lactobacillaceae) were more abundant compared to HC. Lower abundance in both NASH and SS patients compared to HC were confirmed by qPCR for Ruminococcus, Faecalibacterium prausnitzii and Coprococcus. No difference was found between NASH and SS. This lower abundance in NAFLD (NASH+SS) was independent of BMI and IR. NAFLD patients had higher concentrations of fecal propionate and isobutyric acid and serum 2-hydroxybutyrate and L-lactic acid. These findings suggest a potential role for a specific IM community and functional profile in the pathogenesis of NAFLD.

引用

页数：12

共 57 条

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