Investigation of the protective effect of erythropoietin on spinal cord injury in rats

被引：13
作者：
Hong, Zhenghua [1 ]
Hong, Huaxing [1 ]
Chen, Haixiao [1 ]
Wang, Zhangfu [1 ]
Hong, Dun [1 ]
机构：
[1] Taizhou Hosp Zhejiang Prov, Dept Orthoped, Taizhou 317000, Peoples R China
来源：
EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2011年 / 2卷 / 05期
关键词：
platelet-derived growth factor-B; spinal cord injury; erythropoietin; rat; DIABETIC-RATS; PDGF-B; EXPRESSION; RECEPTOR; BRAIN; ACTIVATION; SYSTEM;
D O I：
10.3892/etm.2011.285
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Erythropoietin (EPO) is a promising therapeutic agent used in a variety of spinal cord injuries. Therefore, identifying the specific molecular pathway mediating the neuronal protective effect of EPO after spinal cord injury (SCI) is of great value to the patients concerned. Platelet-derived growth factor (PDGF)-B is an important factor in the recovery of neurological function. We explored changes in the expression of PDGF-B in spinal cord injury rats after receiving EPO treatment. We used a weight-drop contusion SCI model, and EPO treatment group rats received single doses of EPO (1,000 U/kg i.p.) immediately after the operation. Seven days after the operation, the results revealed a more rapid recovery as noted by the higher BBB scores, less disruption and more neuronal regeneration of the spinal cord in the EPO treatment group than that in the SCI group. PDGF-B expression also increased in the EPO treatment group compared to that in the SCI group (P<0.01). This study showed that PDGF-B plays a role in the neuronal protective effect of EPO on spinal cord injury in rats, which may help to explain the quick recovery after EPO treatment of spinal cord injury.
引用
收藏
页码：837 / 841
页数：5
相关论文
共 19 条
[1]   Erythropoietin enhances hippocampal long-term potentiation and memory [J].
Adamcio, Bartosz ;
Sargin, Derya ;
Stradomska, Alicja ;
Medrihan, Lucian ;
Gertler, Christoph ;
Theis, Fabian ;
Zhang, Mingyue ;
Mueller, Michael ;
Hassouna, Imam ;
Hannke, Kathrin ;
Sperling, Swetlana ;
Radyushkin, Konstantin ;
El-Kordi, Ahmed ;
Schulze, Lizzy ;
Ronnenberg, Anja ;
Wolf, Fred ;
Brose, Nils ;
Rhee, Jeong-Seop ;
Zhang, Weiqi ;
Ehrenreich, Hannelore .
BMC BIOLOGY, 2008, 6 (1)
[2]   PDGF-D is a specific, protease-activated ligand for the PDGF β-receptor [J].
Bergsten, E ;
Uutela, M ;
Li, XR ;
Pietras, K ;
Östman, A ;
Heldin, CH ;
Alitalo, K ;
Eriksson, U .
NATURE CELL BIOLOGY, 2001, 3 (05) :512-516
[3]   Activation of the transcription factor NF-κB by the erythropoietin receptor -: Structural requirements and biological significance [J].
Bittorf, T ;
Büchse, T ;
Sasse, T ;
Jaster, R ;
Brock, J .
CELLULAR SIGNALLING, 2001, 13 (09) :673-681
[4]   The PDGF B-chain is involved in the ontogenic susceptibility of the developing rat brain to NMDA toxicity [J].
Egawa-Tsuzuki, T ;
Ohno, M ;
Tanaka, N ;
Takeuchi, Y ;
Uramoto, H ;
Faigle, R ;
Funa, K ;
Ishii, Y ;
Sasahara, M .
EXPERIMENTAL NEUROLOGY, 2004, 186 (01) :89-98
[5]   Neuron-specific ablation of PDGF-B is compatible with normal central nervous system development and astroglial response to injury [J].
Enge, M ;
Wilhelmsson, U ;
Abramsson, A ;
Stakeberg, J ;
Kühn, R ;
Betsholtz, C ;
Pekny, M .
NEUROCHEMICAL RESEARCH, 2003, 28 (02) :271-279
[6]   Erythropoietin and erythropoietin receptor expression after experimental spinal cord injury encourages therapy by exogenous erythropoietin [J].
Grasso, G ;
Sfacteria, A ;
Passalacqua, M ;
Morabito, A ;
Buemi, M ;
Macrì, B ;
Brines, ML ;
Tomasello, F .
NEUROSURGERY, 2005, 56 (04) :821-826
[7]   Neuroprotection by erythropoietin administration after experimental traumatic brain injury [J].
Grasso, Giovanni ;
Sfacteria, Alessandra ;
Meli, Francesco ;
Fodale, Vincenzo ;
Buemi, Michele ;
Iacopino, Domenico G. .
BRAIN RESEARCH, 2007, 1182 :99-105
[8]   The brain erythropoietin system and its potential for therapeutic exploitation in brain disease [J].
Hasselblatt, M ;
Ehrenreich, H ;
Sirén, AL .
JOURNAL OF NEUROSURGICAL ANESTHESIOLOGY, 2006, 18 (02) :132-138
[9]   TSP-1 expression changes in diabetic rats with spinal cord injury [J].
Hong, Zhenghua ;
Chen, Haixiao ;
Hong, Huaxing ;
Lin, Lie ;
Wang, Zhangfu .
NEUROLOGICAL RESEARCH, 2009, 31 (08) :878-882
[10]   GM-CSF inhibits glial scar formation and shows long-term protective effect after spinal cord injury [J].
Huang, Xian ;
Kim, Jin-Mo ;
Kong, Tae Ho ;
Park, So Ra ;
Ha, Yoon ;
Kim, Moon Hang ;
Park, Hyeonseon ;
Yoon, Seung Hwan ;
Park, Hyung Chun ;
Park, Jong Oon ;
Min, Byoung-Hyun ;
Choi, Byung Hyune .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 2009, 277 (1-2) :87-97
12